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Dep. of Plant and Soil Sciences, Oklahoma State Univ., Stillwater, OK 74078
* Corresponding author (bill.raun{at}okstate.edu)
Received for publication January 4, 2007.
ABSTRACT
Long-term experiments play a vital role in revealing dynamic soil and weather processes that directly influence sustainable crop production and ecosystem health. The objective of this review paper is to present one of the oldest long-term continuous winter wheat (Triticum aestivum L.) experiments, the Magruder Plots, and their contribution to the scientific community regarding questions of yield response to amendments, yield stability, percentage organic matter (OM) lost over time, soil nutrient status, microbial activity, weed population, and information on the economic return from winter wheat production. Alexander C. Magruder initiated the experiment in 1892 and is in progress to date. The original plot was started to evaluate wheat production on native prairie soils without fertilization. After 6 yr the principal investigator split the initial plot into two and fertilized one half with cattle manure. The experiment was then modified to include 10 treatments in 1930 by Dr. Horace J. Harper to answer several soil fertility related questions. Since 1947, six treatments have remained intact that evaluate simple combinations of manure, and inorganic N, P, K, and lime. Following 114 yr of continuous winter wheat production under conventional tillage, the check plot that has never received any fertilizer addition continues to produce wheat grain yields of >1 Mg ha–1. Despite the decline in soil organic matter from 4 to 1% during this time period, wheat grain yields continue to show slight increases with time, likely due to improved genetics. While continuous wheat without rotation is not recommended, this 114-yr study documents the feasibility.
Abbreviations: NPKL, sodium nitrate + superphosphate + potash + lime OM, organic matter
LONG-TERM EXPERIMENTS play a vital role in revealing dynamic soil and weather processes that directly influence sustainable crop production and ecosystem health. They are valuable repositories of information in understanding nutrient dynamics and balances along with understanding changes in yield (Davis et al., 2003; Regmi et al., 2002; Mitchell et al., 1991). The importance of long-term experiments lies in their ability to observe trends which may only be seen over long periods of time such as changes in soil OM. Weather, one of the most important factors in crop production, is highly variable and cannot be predicted over a short period of time. According to Grandstedt and Kjellenberg (1997) and Witt et al. (2004), it is not possible to determine with any degree of certainty trends in soil processes or weather changes with short-term studies.
Typical rationale of having long-term experiments would be evaluating changes in soil chemical and physical properties that can be reflected through changes in crop yields. For example, Ladha et al. (2003) were able to track a decline in rice yield at an average rate of 23 kg ha–1 each year using data from 33 long-term experiments. This yield decline was attributed to the loss of OM, the decrease of nutrient supply, and climate fluctuations. While this trend was not universal for all 33 long-term experiments examined, it does indicate that further investigation is needed to develop improved production practices. Similarly, in India during the 1980s, long-term data was used to evaluate the rice–wheat production system efficiency (Bhandari et al., 2002). Based on a 14-yr study in Punjab, it was noted that rice yields declined even when the recommended rates of N, P, and K were applied. This decline was attributed to loss of total soil N and OM. For this reason the continued examination of these long-term experiments is needed to determine if the implementation of these findings can improve yields for these systems. In this review paper we discuss the brief history and procedural evolution of the Magruder Plots, and their significant contribution in untangling several research questions including changes in soil chemical properties and variations in yields; soil microbial and weed dynamics; and varietal performance in relation to fertility gradients on a long-term basis.
HISTORY AND TREATMENT STRUCTURE OF THE MAGRUDER PLOTS
The Magruder Plots, located just off the Oklahoma State University campus in Stillwater, OK, were initiated in 1892 by Alexander C. Magruder to evaluate wheat production on native prairie soils without fertilization (Magruder, 1892, 1893). The land was tilled for the first time in the fall of 1892 with the initiation of the experiment. Since its inception the experiment has experienced several threats of destruction. In the early and late 1930s, campus expansion projects attempted to destroy the Magruder Plots. This effort was culminated thanks to the dedication of researchers responsible for the experiment. In 1947, another expansion project necessitated by increased student numbers threatened the Magruder Plots. The researcher in charge at that time, Dr. Horace J. Harper, fought intensely and the experiment was saved once again; but this time, selected treatments were relocated 1.6 km west of its original location to what is known today as the Agronomy Research Station. The detail of relocation of this experiment in 1947 is documented elsewhere (Chester, 1947; Harper, 1953, 1959). Noteworthy was the physical movement of the surface 40.6 cm of soil from six 30.5- by 6.1-m plots to the new site, comprising >450 metric tons of soil to accomplish the task, and to ensure long-term biological integrity of the experiment. After the relocation, researchers were concerned with a potential threat to the Magruder Plots specifically due to a rumor of the creation of an athletic center where the Plots are located. To preserve the Magruder Plots, researchers in charge in the mid to late 1970s campaigned to enter this long-term experiment into the National Registry of Historic Places and this was successful on 29 Aug. 1979 (Delaporte, 1979).
Only one plot was used to evaluate native wheat production without the application of organic or inorganic fertilizers from 1893 to 1898. The initial objective was to track the productivity of the prairie soils without the addition of any form of fertility reclamation. From 1899 to 1929, half of the experimental area was fertilized with cattle manure while the other half received no fertilization after observing a decline in performance of wheat in the check plot versus the adjacent field (apparently receiving some form of organic fertilizer in the past). In 1930, Dr. Horace J. Harper established 10 separate fertilization treatments on these plots which continued until 1947. Although details are given in the literature (Harper, 1959; Murphy, 1929; Westerman, 1992), it is important to mention how Dr. Harper came up with the new treatment sets which helped in making decisions during the relocation. Dr. Harper had analyzed the soil chemical properties of the Magruder Plots in 1926 and he discovered that P was deficient in the check plots. Additionally, Dr. Harper learned that at the same experiment station, another short-term study revealed that P was the deficient nutrient. He also anticipated that N, K, and lime would be required at some point in the life of the experiment, which was in fact correct. He divided the unfertilized check into five plots and applied superphosphate (P), sodium nitrate + superphosphate (NP), sodium nitrate + superphosphate + potash (NPK), and sodium nitrate + superphosphate + potash + lime (NPKL) in the four plots, each receiving only one treatment set. Similarly, the cattle manure plot was divided into five subplots, of which four were fertilized with P, rock phosphate, PK, and NPK. This treatment structure was continued until 1947. The timeline for changes that occurred in the Magruder Plots and the monthly average rainfall and temperature across 36 yr are given in Table 1 and Fig. 1 . All nonexperimental plot management activities such as weed control and varietal selection have been accomplished as per Oklahoma State University Extension Service recommendation.
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Boman et al. (1996) and Mullen et al. (2001) summarized Magruder Plots yield results in different categories based on changes made throughout the history of the experiment. The yield trend in different treatments was grouped into five categories (1893–1898, 1899–1929, 1930–1957, 1958–1994, and 1995–2006) since the treatment effects were consistent during each of these time periods. The response of winter wheat to treatments since 1995 was consistent with the data averaged for years 1958 to 1994, except the higher yield level for manure, NP, NPK, and NPKL treatments during this period (Fig. 3 ).
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GRAIN YIELD STABILITY IN THE MAGRUDER PLOTS
Stability analysis was initially developed using the dynamic concept (Eberhart and Russell, 1966), in which simple regression is used to assess genotype yield stability from location to location and from year to year. In 1984, this concept was applied to an on-farm variety x N rate experiment in Malawi where the focus was to compare local and composite corn (Zea mays L.) variety performance in different environments (Hildebrand, 1984). Raun et al. (1993) used the concept to evaluate the fertilizer treatment yield stability from year to year, given the large variability observed in the plots. According to Raun et al. (1993) and Guertal et al. (1994), the environment (yearly) mean yield was obtained by averaging yield of all treatments for each year while the mean for each treatment was calculated from subplots. Raun et al. (1993) revealed that when environmental means were <2 Mg ha–1, the cattle manure treated plots performed poorly while the NPK-treated plots had slightly higher yields. The opposite was reported with higher environmental means (>2 Mg ha–1), that is, cattle manure plots had steady yield when growing conditions were optimal (Raun et al., 1993). The check and P plots had the lowest slope coefficients (0.66 and 0.62, respectively), which signifies less-stable yield than the rest of the treatments (Fig. 6 ). The overall lesson was that cattle manure and N fertilizer receiving plots tended to buffer the effect of environmental variability. The practical significance of this analysis is its capacity to reveal treatment effects by reducing the confounding effect of year to year variability that is not evident in conventional ANOVA.
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Recent work by Davis et al. (2003) on the Magruder Plots found that OM decreased 55 to 67% in the last 110 yr, largely a result of cultivation. The highest reduction was in the check (2.41%) and NP (2.61%) plots, while the lowest reduction was in cattle manure (1.85) plots (Table 4). Boman et al. (1996) reported that OM decreased from 4 to 1% in the check plot since the experiment was initiated. The rate of OM decline per year was 0.0151 and 0.0168% in the cattle manure and check plots, respectively (Fig. 7 ). The OM reduction of this magnitude was apparently associated with loss of productivity of arable land; there are some reports that the reduction in OM forced farmers to abandon farming (Wright et al., 2004). Long-term experiments like the Magruder Plots can serve as a warning system by predicting possible changes in the soil ahead of time. One obvious observation in the long-term analysis of soil OM of the Magruder Plots was the reduction of OM level in cattle manure and inorganic NPK plots. This is presumably related to conventional tillage used throughout the life of this long-term experiment. Conventional tillage has been known to deplete soil OM (Wilhelm et al., 2004; Katsvairo et al., 2006). This justification is plausible because researchers showed that turning under and exposing the subsurface soil can break down soil aggregates (Willis, 1955; Hadas, 1990, Edwards, 1991; Beare et al., 1994), indirectly affecting OM level. Destruction of soil aggregates as a result of cultivation alters soil conditions (i.e., temperature, moisture, and aeration), hastening microbial activity and decomposition rate of OM (Rovira and Greacen, 1957; Cambardella and Elliott, 1993). This in turn lowers levels of soil OM that have direct relationships with increased erosion, nutrient leaching, and reduction in biological diversity. Additionally, conventional tillage is known to reduce water use efficiency and decrease water holding capacity of the soil due to decreased OM.
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SOIL NITROGEN, PHOSPHORUS, POTASSIUM, AND pH STATUS
Although several research findings were reported on soil N in this study (e.g., Boman et al., 1996; Fell, 1976), the most comprehensive report on the Magruder Plots was published by Davis et al. (2003). In their paper the authors attempted to estimate soil N balance by quantifying additions and losses in the first 30 cm depth of each of the Magruder Plots (Table 5). The additions were N applied from fertilizer, added to the soil through nonsymbiotic fixation, from atmospheric deposition (mainly rainfall), and mineralization from organic pool. The losses were N removed in the grain, gaseous losses from the plant, denitrified from the soil, and leached from the root zone. Grain N removal for years between 1892 and 2001 for different treatments ranged from 2808 (check) to 4186 kg ha–1 N (cattle manure) with a corresponding average of 25.7 to 38.4 kg ha–1 yr–1 N, respectively. The values were obtained by multiplying yield and percentage N in the grain. The unaccounted N in different treatments ranged from 2 to 13 kg ha–1 yr–1 N after subtracting estimates of losses from additions (Davis et al., 2003). The check had the lowest unaccounted N while the cattle manure treated plots had the highest. Although the objective was to come up with a zero N balance, it did not turn out that way mainly because the inputs for losses and additions were only estimates as reported by the authors. However, the unaccounted N increased as applied N increased regardless of the source (Table 5). Lees et al. (2000) observed similar trends in a study conducted to document unaccounted N using 15N.
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Cattle manure application maintained the pH at the optimum (6.20) level while the application of NP and NPK dropped the pH to 5.10 (Zhang, 1998). Liming, coupled with NPK fertilization, raised the pH to acceptable levels (5.51) although it was not as high as that of the cattle manure treated plots. Apparently, the reduction in pH was associated with N fertilizer; plots that did not receive N fertilizer including the check showed no significant reduction in pH. Information regarding why pH was maintained at optimum levels in the cattle manure plots, even better than the lime-treated plots, was assessed with the data generated from the same experiment. Zhang (1998) attributed this to abundance of basic cations such as Ca found in manure. Parham et al. (2002) indicated that cattle manure enriches some microbial communities that favor higher soil productivity through maintenance of soil pH. Long-term experiments are critical for evaluating the fate of continuous application of organic nutrient sources such as cattle manure, which can contain different concentrations of nutrient and nonnutrient minerals. Edmeades (2003) reviewed 14 field trials of long-term experiments dealing with the use of fertilizers and manures on crop production and soil properties. It was found that fields with cattle manure application had increased levels of OM, P, K, Ca, and Mg in the surface, increased levels of nitrate-nitrogen (NO3–N), Ca, and Mg in the subsoil, and decreased bulk density.
SOIL MICROBIAL ACTIVITY AND SOILBORNE DISEASES
Crucial soil microbial population and community structure information was generated from the Magruder plots (Parham et al., 2003; Sun et al., 2004). It was found that cattle manure enhanced bacterial populations compared with the check plot. From this study, the authors concluded that "The richness and evenness of the bacterial community were enhanced by cattle manure treatments and treatments that included N and P." In another study that assessed the effect of cattle manure application on microbial biomass C and enzyme activity, Parham et al. (2002) found that cattle manure increased microbial activity and stimulated the activity of several enzymes involved in N and P transformation.
Limited information on soilborne disease status was presented in Boman et al. (1996) using data collected in the 1992–1993 crop season. Specifically, the author surveyed the Magruder Plots for lower internode discoloration (%) and amount of wheat germ infected by Pythium spp. The former is essentially an indicator of soilborne disease-causing organisms (Wiese, 1987; Singleton and Russell, 1990). Figure 8 shows that lower internode discoloration, which is a likely symptom of soilborne disease status, was different among the six treatments ranging from 23% in the check plot to 53% in the cattle manure plot (Boman et al., 1996). Alternatively, the Pythium spp. infection of wheat germ was significant among treatments, the check, cattle manure, and NPKL plots had the lowest infection level. The results suggested that in the cattle manure treated plot the abundance of soilborne disease-causing organisms other than Pythium spp. outcompeted the Pythium spp. population. The check had overall lower discoloration and Pythium spp. abundance (Fig. 8). Apparently the soil of the check plot lacked nutrients required by both beneficial and nonbeneficial soil organisms. However, as revealed in the cattle manure plots an increase in beneficial organisms in the soil would likely antagonize and suppress the population of disease-causing pathogens (Hoitink, 1986).
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The Magruder Plots also served to evaluate weed species dynamics and abundance (Banks et al., 1976). In this study they showed that weed species and abundance generally were low in the check plot. With the addition of nutrients in each of the other five treatments, weed species type, total population, and individual species abundance increased, reaching the highest level with the application of NPKL. Exceptions were evening primrose (Oenothera laciniata Hill), carpetweed (Mollago vericillata L.), and henbit (Lamium amplexicaule L.). Evening primrose was abundant in the unfertilized plot, with a decrease in population as fertility levels increased with addition of different types of nutrients. Carpetweed and henbit responded to P and NP fertilization where highest abundance was recorded.
In a way, the Magruder Plots enabled researchers to characterize the weed species in Oklahoma in relation to fertility gradient. These plots were good indicators of a succession trend in a continuous winter wheat cropping system. This could assist Oklahoma producers in devising weed management strategies in continuous winter wheat. A followup study would have confirmed or revealed consistency of weed species dynamics.
THE MAGRUDER PLOTS AS REPOSITORIES OF ECONOMIC RETURN INFORMATION
One aspect of the use of the Magruder Plots was the evaluation of economic returns and reliability of economic yield increases due to treatments. Boman et al. (1996) reported the moving average net return and reliability of economic yield as a function of N, P, and K fertilizer for each 10-yr period. They showed that there was a wide discrepancy in net return due to N fertilization, but mostly positive ranging from $49 to 185 USD ha–1 since 1958. For earlier years, the moving average increase in net return was <0. In contrast, for P the net return was positive for averages computed between 1920 and 1957, and was negative thereafter. Positive increase in net return due to K fertilization was observed only for the moving average computed between 1988 and 1994. This coincided with the apparent response of winter wheat to K fertilization during this period. The peak (>0.8) reliability of obtaining an economic yield increment in response to N, P, and K fertilization was somehow consistent with the increase in net return (Boman et al., 1996).
CONCLUSIONS
The Magruder Plots have proven to be a source of scientific information in sustainable soil fertility management and cereal production. This experiment has helped scientists to monitor long-term changes in soil chemical properties and variations in yields in relation to weather. The information extracted from this experiment on soil microbial and weed dynamics helped researchers and producers to understand possible changes in their farming and ecosystem for better resource management and sustainability. The Magruder Plots have also helped breeders and agronomists to assess varietal performance in relation to fertility gradients and will continue to guide them in future breeding work. The only pitfall of this long-term experiment is the lack of replication, as at its inception the concept of replications and thus statistics was not established. Despite this, measurements taken from the Magruder Plots have contained at least four subsamples, as the plot size is big enough to do so. After all, there are statistical tools today to manage nonreplicated experiments and the issue of replication becomes less important when 100+ years of data are available. The Magruder Plots will continue to be repositories of valuable information for developing sustainable and environmentally friendly winter wheat production systems in Oklahoma and elsewhere. As issues of sustainability and environmental safety become increasingly more important, long-term experiments such as the Magruder Plots will be further explored.
NOTES
Contribution from the Oklahoma Agricultural Experiment Station.
REFERENCES
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