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a Dep. of Agronomy, Iowa State Univ., Ames, IA 50011
b USDA-ARS National Soil Tilth Lab., Ames, IA 50011
* Corresponding author (lgibson{at}iastate.edu)
Received for publication July 3, 2006.
| ABSTRACT |
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Abbreviations: GDD, growing degree days
| INTRODUCTION |
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Reducing N loss from current cropping systems in the U.S. Corn Belt will require crops that can absorb N efficiently (Dhugga and Waines, 1989; Blankenau et al., 2002), use the N effectively to cut down fertilizer requirements (Huggins and Pan, 1993), and optimum timing of fertilizer that is applied (Melaj et al., 2003). More widespread introduction of winter triticale into current corn and soybean production systems could provide the type of crop management needed to minimize N loss to surface and groundwater, maintain or improve soil quality, and improve economic return to farmers (Katsvairo and Cox, 2000; Dinnes et al., 2002; Bundy and Andraski, 2005).
To encourage adoption of triticale, optimum N fertilization rates must be determined to avoid excess expenditure on N fertilizer and limit excess residual NO3 from accumulating in the soil profile (Blankenau et al., 2002; Follett and Delgado, 2002) and subsequently leaching into surface and groundwater resources (Jaynes et al., 2001; Strock et al., 2004; Kocyigit, 2004). Efficient N use in a cropping system has the potential to reduce NO3 losses while optimizing grain yield and quality, but this is dependant on the producer's ability to correctly meet crop N requirements (Stockdale et al., 1997).
Proper cultural techniques, including N management, are important for obtaining optimum crop yields and positive economic returns while limiting negative environmental impacts of crop production. Schwarte et al. (2005) reported that planting date plays a key role in the productivity and N capture of winter triticale in Iowa. Planting in mid- to late September maximized triticale grain and forage yields when compared with October planting. Total N removal in aboveground dry matter ranged from 52 to 161 kg ha1 depending on season, location, and planting date. In one of two seasons, N accumulation was 37% greater for mid-September planted triticale than mid-October planting. There was no difference in N accumulation among planting dates during the other season. In both years, >50% of triticale N uptake occurred by mid-May and nearly 75% of N uptake had occurred by late May. Triticale thus appeared to be efficient in capturing N during the early spring when it is most likely to leach because of high rainfall.
Winter triticale can provide feed, forage, grain, and straw products; reduce environmental problems associated with current cropping practices; and offer other tangible benefits. Before winter triticale will be widely adopted as a crop option in the midwestern USA, several important questions regarding its culture and management must be answered. Nitrogen management for optimum triticale productivity is one of the most important among these key questions. The objectives of this study were to determine the quantity of N fertilizer needed to optimize winter triticale dry matter production and grain yield at two Iowa locations and to estimate the amount of N taken up by a triticale crop following soybean or corn silage as the previous crop. Providing this knowledge will enable farmers and others to make better decisions regarding the economic and environmental benefits of triticale and improve management decisions regarding triticale production and use.
| MATERIALS AND METHODS |
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Crop Culture and Nitrogen Application
Corn silage and soybean crops were grown before winter triticale at both sites. Fields in Ames were prepared for corn and soybean planting with one pass of a field cultivator. No preplant tillage was used at Lewis. The corn and soybean were planted in alternating strips, 9.15 m wide (12 rows with 0.762 m between rows) and 21.34 m long. An early maturity group soybean was grown at both sites to ensure an optimum triticale planting date (Schwarte et al., 2005). Corn was harvested as silage and soybean was harvested as grain with the residue returned to the field. Dates of important field operations and sampling activities are listed in Table 1.
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At Ames in 2003, corn (Dekalb DKC6411 RR, 114-d relative maturity) was planted at 79535 seeds ha1 and soybean (Dekalb DKB1751 RR, 1.7 relative maturity) was planted at 395200 seeds ha1 on 20 May. For Ames in 2004, the same corn hybrid and soybean cultivar were planted at the same densities on 5 May. Nitrogen fertilizer was applied to corn at 134 kg N ha1 in the form of urea on 9 June 2003 and in the form of injected (320 g kg1 or 32%) ureaNH4NO3 solution on 16 June 2004. The corn was cultivated between the rows on 9 June 2003. Roundup Ultramax (isopropylamine salt of glyphosate, [N-phosphonomethyl glycine]) was applied to the soybean and corn at 1.9 L ha1 on 16 June 2003. Roundup Weathermax was applied to the soybean and corn at 1.8 L ha1 on 15 June 2004.
At Lewis in 2003, corn (Channel 7699C, 109-d relative maturity) was planted at 79 040 seeds ha1 on 27 April and soybean (Pioneer 92B05 RR, 1.9 relative maturity) was planted at 395200 seeds ha1 on 13 May. In 2004, corn (Nutrident C 1153 ND, 115-d relative maturity) was planted on 19 April and soybean (Pioneer 92B05 RR, 1.9 relative maturity) was planted on 23 April. The same planting densities were used in 2004 and 2003. Weed management in 2003 consisted of 55 mL ha1 Steadfast [Nicosulfuron (2-[[(4,6-dimethoxypyrimidin-2yl)aminocarbonyl]aminosulfonyl]-N,N-dimethyl-3-pyridinecarboxamide)], 7.7 L ha1 Callisto [mestotrione (2-[4-(methylsulfonyl)-2-nitrobenzoyl]-1,3-cyclohexanedione)], 1.1 kg ha1 atrazine [6-chloro-N-ethyl-N'-(1-methylethyl)-1,3,5-triazine-2,4-diamine] applied to corn on 7 June and 1.6 L ha1 Roundup Weathermax applied to soybean on 20 June. Weed management in 2004 consisted of interrow cultivation of corn on 15 June and 1.5 L ha1 Roundup Weathermax applied to soybean on 15 June.
Winter triticale (DANKO Presto in 2003, NE426GT in 2004) was seeded at 320 seeds m2 with a Tye Model 2007 no-till drill (AGCO Corp., Lockney, TX). The release of NE426GT in 2004 provided better adapted triticale genetics for this study (Baenziger et al., 2005). The row spacing was 20.3 cm. No tillage was performed between corn or soybean harvest and triticale planting at either site.
Four N fertilizer rates (0, 33, 66, or 99 kg N ha1) were assigned to each pair of triticale plots growing on the harvested corn silage and soybean strips. The N fertilizer was applied at Ames using a Gandy Model 1010T-TBM spreader (Gandy Co., Owatonna, MN) with a 3-m width and at Lewis with a Gandy Model 6500 spreader with a 1.5-m width. Ammonium nitrate was used as the N fertilizer source at both locations.
Plant Measurements
Corn silage for the entire sites was harvested with a forage chopper and soybean was combine harvested. Triticale dry matter production was determined every 3 wk (four times) in the spring starting with the first week of May (Table 1). The aboveground plant material from a 48.3-cm length of row was harvested from two randomly selected areas within each plot. The two samples for each plot were pooled, oven dried at 65°C for at least 48 h, and weighed. A subsample was taken after weighing and ground to pass a 2-mm screen using a Thomas-Wiley mill (Model 4, Thomas Scientific, Swedesboro, NJ). The sample was ground a second time using an Udy cyclone sample mill (Udy Corp., Ft. Collins, Co) to pass a 0.5-mm screen. These ground samples were analyzed by dry combustion in a Fison NA 1500 Elemental Analyzer (Fison Instruments SpA, Milan, Italy) to determine total N concentration of the harvested dry matter (AOAC International, 2000). Total dry matter N was calculated for each sample by multiplying dry matter by N concentration. Apparent fertilizer N uptake efficiency was calculated at maturity as
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Triticale grain was harvested with combines equipped with onboard electronic weighing systems. The harvested area in each plot was 3.66 m wide by 21.34 m long at Ames and 4.57 m wide by 21.34 m long at Lewis. Triticale grain subsamples (approximately 2000 g) were collected to determine moisture content, 1000-kernel weight, and test weight. Crop residue and other debris were removed from the grain samples with a seed cleaner (Office Model Clipper, Ferrel Ross, Bluffton, IN). A thousand seeds were counted with an electronic seed counter (Model 850-2, Old Mill Co., Savage, MD) and weighed. Moisture content and test weight of triticale grain were determined on the cleaned grain using a grain analysis computer (Model GAC2100, Dickey-John, Auburn, IL). Final triticale grain yields were adjusted to 135 g kg1 moisture content. The triticale grain was ground to a fine powder using a Magic Mill III Plus grain mill (K-Tec, Orem, UT) and analyzed for moisture using AACC Method 44-15A (American Association of Cereal Chemists, 2003) and total N concentration by dry combustion (AOAC International, 2000) in a Fison NA 1500 Elemental Analyzer (Fison Instruments SpA, Milan, Italy).
Spikes per square meter and kernels per spike were measured using samples collected the day of combine harvest. The number of spikes per square meter was determined by sampling 48.3 cm of row from two areas of each plot (0.2 m2) and counting the total number of spikes. Kernels per spike were determined by harvesting 10 consecutive spikes from two areas within each plot and counting the kernels threshed from the 20 spikes. Straw samples were collected from the swath created by the combine.
Weather Data
The daily minimum temperature, maximum temperature, and rainfall were recorded for 2003, 2004, and 2005 using weather stations at each location. The mean weather conditions for each site were determined using means from 1951 to 2005 from the Iowa Environmental Mesonet (2006). Daily rainfall measurements did not include frozen precipitation, which was not measured. Growing degree days (GDD, 0°C base temperature) were calculated using the equation
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Statistical Design and Analysis
The statistical design for these field experiments was a randomized complete block with four replications and separate analyses for each location and previous crop combination. The corn and soybean strips that preceded the triticale crops were not randomized because of the difficulty it would have created for managing and harvesting those crops. This lack of randomization required separate statistical analysis for each previous crop. The variance for each factor measured was stabilized through transformation according to the procedure of Box and Cox (1964). Analysis of variance was performed on the transformed data using the GLM procedure of SAS (SAS Institute, 2006). Too few years were included in the experiment to test it as a main effect (Gomez and Gomez, 1984, p. 328332), so a combined analysis was performed over years. Main effects of N rate and the N rate x year interaction were analyzed using an F test. The F test for N rate was calculated using the mean squares for the year x N rate interaction. The F test for the N rate x year interaction was calculated using the error mean square. Tukey's test was used to make mean comparisons at the P
0.05 level (Steel and Torrie, 1980). Tables and figures contain data combined for the 2 yr of the study. In the few instances where N rate x year interactions occurred, they are discussed in more detail.
Temporal changes in dry matter accumulation, N concentrations, and N accumulation in the spring and summer were analyzed with regression and ANOVA techniques. The data for four sampling dates in each year were converted from calendar time to thermal time (GDD). Regression analysis was used to fit a line to the four measured data points for each plot. A second-order polynomial line was fit to dry matter and N accumulation responses to thermal time. A type III exponential function (Sit and Poulin-Costello, 1994) was used to fit N concentration response to thermal time. Predicted values were calculated for each 200-GDD interval by solving the regression equations. The predicted values at each 200-GDD increment were compared for statistical significance at the P
0.05 level using ANOVA. Regression equations were developed for the main effects of each N rate and plotted for visualization of the responses.
| RESULTS |
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There were no significant differences in triticale dry matter among the four N rates for either previous crop at Lewis. Similar to Ames, the lowest N concentration at Lewis was associated with 0 kg N ha1 regardless of the previous crop. Nitrogen concentration and uptake of triticale grown after corn silage at Lewis increased with the addition of N up to 66 kg ha1, but weren't increased any further with 99 kg ha1. Nitrogen concentration of triticale grown after soybean at Lewis increased with each incremental addition of N. There was a year x N rate interaction for N uptake by triticale grown after soybean at Lewis. In 2004, the N uptake increased with each incremental addition of N fertilizer (data not shown). In 2005, there were no differences in N uptake by triticale for the four different N rate treatments.
Triticale Grain Yield, Grain Quality, Lodging, and Straw Nitrogen Concentration
Applying 33 kg N ha1 increased triticale grain yield after corn silage at Ames by 64% compared with 0 N (Table 3). Nitrogen applications of 66 and 99 kg ha1 produced grain yields similar to 33 kg ha1. Of the three yield-determining components, spikes per square meter and seeds per spike were increased by application of N fertilizer to triticale grown after corn silage at Ames. There was a year x N rate interaction for kernel weight of triticale grown after corn silage at Ames. This interaction occurred because kernel weights were 2 mg less for 99 kg N ha1 than for 0 or 33 kg N ha1 in 20032004. There were no differences in kernel weight among the N rates in 20042005. Triticale grain yield after soybean at Ames was increased 24% when N rate was increased from 0 to 33 kg ha1. However, there was no difference in grain yield for the 0, 66, and 99 kg N ha1 rates or the 33, 66, and 99 kg ha1. Seeds per spike was the only yield component significantly increased with the addition of N after soybean at Ames. Triticale grain yield and yield components did not respond to N application after corn silage or soybean at Lewis. The 20032004 triticale crop was affected by Septoria leaf blotch (Septoria spp.) at both sites due to the cool, moist conditions during June and July (Wiese, 1987, p. 4345). This disease was presumably a major factor responsible for low grain yields in 2004 (data not shown).
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Apparent fertilizer N uptake efficiency for winter triticale did not vary with N rate and was 88% after corn silage at Ames, 91% after soybean at Ames, 100% after corn silage at Lewis, and 61% after soybean at Lewis.
| DISCUSSION |
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Winter Triticale as a Forage Crop
Management and harvest timing of forage crops is a balance between yield and quality of the aboveground plant matter (Collins and Fritz, 2003). A common goal of forage producers is to harvest the greatest forage quantity possible while keeping forage nutritive value at levels needed to optimize performance of the type and class of livestock being fed (Collins and Fritz, 2003). In our study, an increase in triticale forage dry matter occurred with the first 33 kg ha1 N fertilizer addition at Ames. Dry matter differences from N fertilization rate differences were not statistically significant at Lewis. The greatest N concentration levels, however, were generally produced with 99 kg N ha1 at both Ames and Lewis, but N concentrations at fertilization levels above 99 kg ha1 were not tested. These results suggest 33 kg ha1 was optimum for forage yield, but N levels above 33 kg ha1 were needed to produce forage with the greatest N concentration.
Fit of the triticale forage crop within a cropping system is an additional consideration in the north central USA, where winter cereals are often planted immediately after corn silage or soybean harvest in September, cut for forage in mid- to late May, and followed by corn silage or soybean. Greater than 95% yield potential is obtained in Iowa by planting corn before 15 May (Farnham, 2001) and soybean before 25 May (Whigham et al., 2000). When making plans for growing and harvesting triticale forage, it can be useful to determine the amount of dry matter and the N concentration of the dry matter at these dates. For the 2 yr of this study, average GDD accumulated after 1 March (base 0°C) at Ames were 709 for 15 May and 881 for 25 May. Average GDD accumulated after 1 March (base 0°C) at Lewis were 764 for 15 May and 956 for 25 May.
At 709 GDD and fertilizer rates of 33 kg N ha1 or greater near Ames, 5.5 Mg ha1 triticale dry matter had been produced after corn silage with N concentration of 143 to 186 g kg1. The lower N concentration was produced with 33 kg ha1 N fertilizer, whereas the greater N concentration was produced with 99 kg N ha1. Triticale dry matter produced after soybean was 7.4 Mg ha1 with N concentration of 139 to 188 g kg1. At 881 GDD, 6.7 Mg ha1 dry matter had been produced after corn silage with N concentration of 115 to 148 g kg1 and 9.2 Mg ha1 dry matter were produced after soybean with N concentration of 112 to 150 g kg1.
At 764 GDD and fertilizer rates of 33 kg N ha1 or greater near Lewis, 7.2 Mg ha1 dry matter had been produced after corn silage and 7.9 Mg ha1 dry matter had been produced after soybean. Nitrogen concentration was 204 to 246 g kg1 after corn silage and 182 to 201 g kg1 after soybean. The lower N concentration was produced with 33 kg ha1 N fertilizer, whereas the greater N concentration was produced with 99 kg N ha1. At 956 GDD, 9.2 Mg ha1 dry matter had been produced after corn silage and 10.3 Mg ha1 dry matter had been produced after soybean. Nitrogen concentration was 157 to 191 g kg1 after corn silage and 147 to 161 g kg1 after soybean.
Winter Triticale as a Grain Crop
Winter triticale is harvested as a grain crop during the second or third week of July in Iowa. Maximum grain yields in our study were 3.5 Mg ha1 after corn silage and 4.1 Mg ha1 after soybean at Ames, and 3.0 Mg ha1 after corn silage and 3.6 Mg ha1 after soybean at Lewis. Maximizing triticale grain yield after both corn silage and soybean at Ames required 33 kg N ha1. At Lewis, maximum grain yields were produced with no addition of N fertilizer. Nitrogen fertilization levels above 33 kg ha1 were detrimental because they increased the potential for lodging and lower test weight grain. Addition of winter triticale as a grain crop into a cornsoybean rotation could be beneficial for limiting N loss since maximum grain yields were produced with N fertilizer rates of 33 kg ha1 or less. This was less than the 45 to 65 kg N ha1 required to maximize grain yield in continuous wheat (Triticum aestivum L.) in Oklahoma (Raun and Johnson, 1995) and the 70 to 128 kg N ha1 needed to maximize winter wheat yields after soybean and grain sorghum [Sorghum bicolor (L.) Moench] in Kansas (Staggenborg et al., 2003). Apparent fertilizer N uptake efficiencies of the winter triticale in our study were 60 to 100%, which were greater than the 20 to 65% reported for winter wheat in south central Texas (Alcoz et al., 1993) and 50 to 60% for winter wheat in Europe (Blankenau and Kuhlmann, 2000; Blankenau et al., 2000).
Winter Triticale as a Cover Crop
A major benefit of winter triticale, regardless of use, is its ability to capture residual soil NO3 from the production of previous crops. Maximum winter triticale forage and grain yields in this study were produced with N fertilizer applications of 33 kg ha1. Average N uptake by triticale was 107 kg ha1 when 33 kg N ha1 was applied as fertilizer and 146 kg ha1 when 99 kg N ha1 was applied. These results indicate that winter triticale grown after corn silage or soybean can capture a considerable amount of N beyond that applied as fertilizer. Residual N from production of the previous corn and soybean crops was a probable source of N captured by the triticale crop (Kessavalou and Walters, 1999; Strock et al., 2004).
Lack of satisfactory economic return is a common problem with the use of winter cover crops (Randall and Mulla, 2001; DeBruin et al., 2005). Although a complete economic analysis of winter triticale production is beyond the scope of our current study, winter triticale holds promise for providing the positive N capture benefits of a rye cover crop, while simultaneously expanding the crop rotation and producing valuable forage or grain for use in livestock rations.
Nitrogen uptake by winter triticale in the current study was mostly complete by the first sampling in early May, which corresponded with the early stages of stem elongation, regardless of N rate. This differs from the results of Schwarte et al. (2005), who found triticale accumulating N in Iowa up until anthesis in early June. More than 60% of the N, however, had accumulated in winter triticale shoots by early May in the study by Schwarte et al. (2005). Differences in cultivars, planting date, seasonal weather patterns, soil N status, and rate of GDD accumulation are all factors that may have contributed to this difference in N uptake. Apparent fertilizer N uptake efficiencies near 100% and N uptake of
109 kg N ha1 when 99 kg N ha1 was applied, however, suggest that plant-available N was mostly depleted from the soil by early May in the current study. This limited plant N uptake after early May. Past research with wheat suggests that winter triticale could continue assimilating N after early May if it is available to the crop. Nitrogen fertilizer applied as late as the boot stage (Alcoz et al., 1993; Lloveras et al., 2001) and anthesis (Wuest and Cassman, 1992) was assimilated into wheat. Since N uptake in the current study was complete by early May, harvest as a forage crop in mid- to late May would have resulted in N uptake similar to that obtained with grain harvest and straw removal.
Winter triticale could reduce soil erosion by providing significant surface cover during spring. Kessavalou and Walters (1997) found an average of 1.4 Mg ha1 rye (Secale cereale L.) dry matter yield in combination with soybean residue in a cornsoybeanrye cover crop rotation resulted in surface residue cover comparable to that of corn residue in a continuous corn system. In our study, dry matter in early May exceeded 3 Mg ha1 in the 0 kg N ha1 treatment for winter triticale after both corn silage and soybean at Ames and Lewis.
| CONCLUSIONS |
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| ACKNOWLEDGMENTS |
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| NOTES |
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| REFERENCES |
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