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a The Noble Foundation, 2510 Sam Noble Pkwy., Ardmore, OK 73401
b Texas Agric. Exp. Stn., Texas A&M Univ. Research & Extension Center, 1229 N. Hwy. 281, Stephenville, TX 76401
* Corresponding author (tjbutler{at}noble.org)
Received for publication December 20, 2005.
| ABSTRACT |
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Abbreviations: CEC, cation exchange capacity CP, crude protein DM, dry matter EC, electrical conductivity EDTA, ethylenediaminetetraacetic acid OM, organic matter
| INTRODUCTION |
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The use of compost promotes soil aggregation, which improves soil structure, increases soil pH, benefits water infiltration rate, and improves water-holding capacity (Murray, 1981; USDA NRCS, 2004). The NPK percentages of finished compost are relatively low, but their benefit lies in the slow release of organically-bound N and P in the soil that plants can use more effectively (Gershuny and Martin, 1992). Composting reduces the amount of weeds seeds due to the heat generated during the process and NO3N tends to be more stable in compost, when compared to raw manure (Helton, 2004).
Forage P uptake from soils is highly variable and is a direct function of soil P content, soil physical properties, forage biomass, and forage P concentration, the latter often species-specific (Pierzynski and Logan, 1993). The application of composted dairy manure to soils can increase plant P concentrations and forage yields resulting in greater P removal, a phenomenon observed when compost is applied to summer annual dicots (Muir et al., 2001a), annual monocots (Muir et al., 2001b), or warm-season perennial grasses (Sanderson and Jones, 1997). The efficacy of using composted dairy manure on cool-season perennial grasses, however, has not been investigated in north-central Texas.
Tall wheatgrass is a cool-season perennial grass that may have potential to provide forage of high nutritive value during the winter months, when the dominant warm-season grasses are dormant (Lauriault et al., 2002). Dotzenko (1961) and Undersander and Naylor (1987) reported Jose tall wheatgrass DM yields of 10 800 and 11 300 kg ha1 under irrigation, however, Malinowski et al. (2003) and Gillen and Berg (2005) reported only 900 to 3600 kg DM ha1. Malinowski et al. (2003) reported greater yields of Jose tall wheatgrass when clipped at stubble height of 15 cm compared to 7.5 cm the second growing season due to stand decline, and Gillen and Berg (2005) reported that Jose tall wheatgrass stands declined less rapidly when stubble height was 15 cm compared to 10 cm. A cool-season perennial grass could have an advantage over cool-season annual grasses since it does not have to be replanted each autumn, and soil erosion should be reduced because of perennial cover (Gillen and Berg, 2005). Typically soils in this region are very low in available P (Muir et al., 2001a). Composted dairy manure could serve to correct this deficiency. To assess the potential of tall wheatgrass in this environment where P availability is limiting, its P fertility requirement and uptake needs to be determined.
Two soil P testing methods, historically used to measure soil P for both agronomic and regulatory purposes, ammonium acetate-ethylenediaminetetraacetic acid (NH4OAc-EDTA) (Hons et al., 1990) and Mehlich-3 (Mehlich, 1984), can vary considerably in their estimation of plant-available soil P, when P fertilizer (triple superphosphate) is applied (T.J. Butler, unpublished data, 2006). However, it is still unclear which soil testing method should be used to predict available soil P when composted manure is applied. The objectives in this study were to: (i) study the effect of composted dairy manure on selected Windthorst soil characteristics, (ii) evaluate two soil testing methods for measuring P in a Windthorst soil receiving composted dairy manure, and (iii) determine tall wheatgrass yield response, nutrient concentrations, and nutrient removal rates with six rates of composted dairy manure and two rates of inorganic N fertilizer.
| MATERIALS AND METHODS |
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In the second (20022003) and third (20032004) growing seasons, a portion of plot area (1 by 7 m) was harvested 15 cm above soil surface (Malinowski et al., 2003; Gillen and Berg, 2005) with an Almaco small-plot harvester (Almaco, Nevada, IA) three times (December, March, and May), when tall wheatgrass in the control (zero compost) plots were 25 to 30 cm tall. Subsamples were used to determine forage DM yield by drying approximately 400 g of plant material in a forced-air oven at 55°C until weight loss ceased. Total yearly aboveground DM production was estimated by totaling all yields from each subplot. Representative forage subsamples from each subplot and year were ground through a Wiley mill (Thomas-Wiley Co., Philadelphia, PA) equipped with 1-mm screen. Nitrogen in the forage was determined with a micro-Kjeldahl procedure (Sweeney, 1989), while the minerals (P, K, and S) were digested in nitric acid (Havlin and Soltanpour, 1989) and analyzed using a Technicon Autoanalyzer II (Technicon Industrial Systems, Tarryton NY). Nitrogen concentration was multiplied by 6.25 and reported as CP (Van Soest, 1994). Concentrations of these plant components are reported as season-long weighted averages for each subplot. Nutrient removal was estimated by multiplying the nutrient concentration and the DM yield.
Approximately 15 soil cores were taken to a 15-cm depth at the end of each growing season and composited by subplot to determine treatment differences. Soils were analyzed for pH using 1:2 ratio of soil to deionized water (Schofield and Taylor, 1995), electrical conductivity (EC) (U.S. Salinity Laboratory Staff, 1954), NO3N by Cd reduction (Keeney and Nelson, 1982), and P, K, S, Na, Mg, and Ca based on two soil-extractant methods, acidified NH4OAc-EDTA (Hons et al., 1990) and Mehlich-3 (Mehlich, 1984). Elements in both extractants were measured using inductively coupled argon plasma optical emission spectrometer (ICPOES) (Spectro Radial Modula ICP, Spectro Analytical Instruments, Marlborough, MA). Soil OM was determined by using the Loss-On-Ignition Method (LOI) (Nelson and Sommers, 1996). Infiltration rate of water into the soil was measured with a Turf-tec double ring infiltrometer (Coral Springs, FL), by averaging three readings from each subplot at the end of each growing season. The infiltration rate was determined as the amount of water per surface area and time unit which penetrated the soil (Bouwer, 1986).
Data were subjected to analyses of variance using PROC GLM (SAS Institute, 1999) with treatment differences less than P = 0.05 reported as significant. Means, where appropriate, were separated using Fisher's Protected LSD test at P = 0.05 level of significance. Polynomial contrast (PROC GLM) and regression analysis (PROC REG) (SAS, 1999) were also used on all variables to determine response curves related to compost application.
| RESULTS AND DISCUSSION |
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Soil Organic Matter
Year, year x compost rate, year x N rate, compost x N, and year x compost x N rate interactions were not significant (P > 0.05) for soil OM, therefore data are pooled across years. Although the OM in the composted dairy manure was only 150 g kg1, soil OM increased (P < 0.001) curvilinear (r2 = 0.37) as composted dairy manure rates increased (Table 1). Soil OM in the untreated plots averaged 13 g kg1 compared to 20 g kg1 in the plots with the greatest compost rate (54% increase). This relationship is described by:
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Soil Infiltration Rate
Year, year x compost rate, year x N rate, compost x N, and year x compost x N rate interactions were not significant (P > 0.05) for soil infiltration rate, therefore data are pooled across years. Soil infiltration rate increased (P < 0.001) curvilinear (r2 = 0.92) with increasing rates of composted dairy manure (Table 1). Infiltration rate increased by 100, 242, 292, 408, and 550% for 11.2, 22.4, 44.8, 89.6, and 179.2 Mg ha1, respectively, when compared to the control. This relationship is described by:
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Soil Nitrate Nitrogen
Year, year x compost rate, year x N rate, compost x N, and year x compost x N rate interactions were not significant (P > 0.05) for soil NO3N, therefore data are pooled across years. Composted dairy manure had little effect on soil NO3N levels (P > 0.05), although there was a numeric trend for greater composted manure rates having lower soil NO3N levels, which could be related to greater forage DM yields and N removal from those plots (Tables 1 and 2). Composting dairy manure tends to lower NH4N levels compared to the original manure, but NO3N tends to be more stable in compost. In at least one study looking at both dairy manure and its compost, however, NH4N concentrations were more stable in the soil than was NO3N (Helton, 2004). For the inorganic rate treatment, soil NO3N level increased (P < 0.01) by 41% at the 336 kg N ha1 rate (41 mg kg1) compared to 224 kg N ha1 (29 mg kg1).
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The Texas Cooperative Extension Soil, Water and Forage Testing Laboratory adopted the statewide use of the Mehlich-3 method in January 2004, following the determination that the NH4OAc-EDTA method dissolved nonplant-available apatite in certain calcareous soils (T. Provin, personal communication, 2004). It appears that the Mehlich-3 extractant is suitable for this acid soil receiving large amounts of composted dairy manure. Inorganic N rate had no effect on plant-available soil P.
Soil Potassium
Year, year x compost rate, year x N rate, compost x N, and year x compost x N rate interactions were not significant (P > 0.05) for soil K, therefore data are pooled across years. Soil K levels also increased (P < 0.001) linearly (r2 = 0.60) with both extractants as compost rate increased (Table 1), a phenomenon that was also observed in other compost studies (Schlegel, 1992; Helton, 2004); however, the extractants differed in their estimation of plant-available K (P < 0.0001). These relationships are described by:
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Tall Wheatgrass
Sulfur concentrations of tall wheatgrass tissue and S levels of soil did not differ (P > 0.05) among composted dairy manure and inorganic N treatments, therefore S removal rates are not reported, since these differences were attributed to DM yield differences between years.
Forage Dry Matter Yield
Year and year x compost rate interactions were significant (P < 0.05) for DM yield, therefore data are reported by year. Forage DM yields were greater (P < 0.001) in the 20022003 growing season (Table 2), possibly due to greater early-season rainfall (OctoberDecember), even though total rainfall among growing seasons (SeptemberJune) did not differ (Fig. 1
). Precipitation totaled 782 and 787 mm in the 20022003 and 20032004 seasons, respectively, however in 20032004, a greater portion of the rainfall occurred at the end of the season (AprilJune). Lauriault et al. (2002) reported that in one growing season Jose tall wheatgrass responded more efficiently to 168 kg N ha1 when rainfall occurred before December compared to a single irrigation in mid-January or two irrigations in mid-December and mid-February.
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In this study, forage yields did not differ (P > 0.05) between inorganic rates of N fertilizer (224 and 336 kg N ha1), which indicates that N was not the limiting factor for plant growth; however further study is needed to determine the optimal N rate in this environment. In a N rate study applying 0 to 720 kg N ha1, Dotzenko (1961) reported that Jose tall wheatgrass yield peaked at 360 kg N ha1, which yielded 10800 kg DM ha1 under irrigation. Yield varies depending on rainfall amount and distribution as well as other environmental conditions in relation to how well adapted the species is to that environment. In this study, tall wheatgrass responded positively to compost dairy manure, which improved soil fertility, especially soil P. Based on these results, tall wheatgrass has forage potential for the region, and could have an advantage over cool-season annual forage grasses since it does not have to be replanted each year.
Crude Protein and Nitrogen Removal
Year and year x compost rate interactions were apparent (P < 0.05) for CP and N removal, therefore data are reported by year. Forage CP concentration was greater (P < 0.001) in 20022003 (ranging from 207231 g kg1) compared to 20032004 (158175 g kg1) (Table 2); however, all CP values would be considered adequate for most livestock classes (Ball et al., 2002). Crude protein was greatest (P < 0.05) at the two highest compost rates (89.6 and 179.2 Mg ha1) in 20022003 but did not differ (P > 0.05) among compost rates in the 20032004 growing season (Table 2). The increase in CP in 20022003 with the highest compost rate is surprising since the two inorganic N fertilizer rates did not differ in CP that season. One explanation could be that the organic N from the composted dairy manure was taken up more effectively than the inorganic N, but this is speculative. These relatively high values are similar to studies that harvested immature forage and are greater than values reported for more mature cool-season perennial grasses. Read (1979) reported up to 230 g CP kg1 for immature cool-season perennial grasses in north Texas while Gillen and Berg (2005) reported that CP of Jose tall wheatgrass declined from an average of 211 g kg1 in April, 172 g kg1 in May to 137 g kg1 in June, and Hall (1998) reported that other perennial cool-season grasses showed a marked decline in CP values as harvest interval increased, with values that varied from 117 g kg1 for 70-d intervals to 190 g kg1 for 35-d intervals. In this study, CP values did not differ (P > 0.05) between the two inorganic N rates in 20022003. However, CP was 10% greater (P < 0.05) with the 336 kg N ha1 rate in 20032004, but this is probably not of biological importance.
The amount of N removed, which is a function of N concentration and DM yield, followed a similar trend to that of yield. In 20022003, N removal was greater (P < 0.001) than the 20032004 growing season, primarily due to the increased DM production associated with greater rainfall occurring early in the season. In both seasons, the amount of N removed also increased (P < 0.001) curvilinear (r2 = 0.70 and 0.41, respectively) as compost rate increased. The amount of N removed increased up to 119%, ranging from 161 to 353 kg N ha1 in 20022003 and up to 72% increase in 20032004, ranging from 98 to 169 kg N ha1 (Table 2). Inorganic N rate did not affect (P > 0.05) N concentration or N removal in 20022003, however in 20032004, the 336 kg N ha1 rate removed 17% more N (P < 0.05) due to 10% greater concentration of N in the forage tissue. These data illustrate that the amount of nutrient removal is closely related to DM yield, and as yield increases, the nutrient removal rate also increases.
Phosphorus Concentration and Phosphorus Removal
Year and year x compost rate interactions were significant (P < 0.05) for P concentration and P removal, therefore data are reported by year. Forage P concentrations (Table 2) in 20022003 ranged from 1.8 to 2.8 g P kg1, which was greater (P < 0.001) than in 20032004 (ranging from 1.42.3 g P kg1). This greater concentration in 20022003 could be attributed to more soil P being available from the compost applications made at establishment, while less P was available the following growing season. In the 20022003 season, tall wheatgrass P concentrations increased (P < 0.001) linearly (r2 = 0.37) by 6 to 56% from the lowest to highest compost rate, and in the 20032004 season it increased (P < 0.001) curvilinear (r2 = 0.72) by 14 to 64%. These P concentrations are similar to those reported elsewhere. Undersander and Naylor (1987) reported that Jose tall wheatgrass averaged 1.9 g P kg1 when harvested biweekly, and ranged from 1.4 to 2.2 g P kg1.
The amount of P removed was greater (P < 0.001) in the 20022003 growing season compared to the 20032004 season, primarily due to greater forage yields and P concentrations resulting from compost application (Table 2). The amount of P removed increased (P < 0.001) as compost rate increased, ranging from 8.7 to 26.7 kg P ha1 in 20022003 and from 5.4 to 14.0 kg P ha1 in 20032004. In both growing seasons, removal of P increased (P < 0.001) curvilinear (r2 = 0.78 and 0.83, respectively) as compost rate increased by 40 to 206% in 20022003 and by 50 to 159% in 20032004 (Table 2). However, the cumulative P recovery rates (combined P removal for both growing seasons) were 0.141, 0.106, 0.084, 0.058, 0.038 for composted manure rates of 11.2, 22.4, 44.8, 89.6, 179.2 Mg ha1, respectively, indicating that P efficiency is greater at lower compost rates. Inorganic N fertilizer rate had no effect (P > 0.05) on P concentration or the amount of P removed from the soil. These data indicate that tall wheatgrass could potentially be used on high P soils to remove excess P.
Potassium Concentration and Potassium Removal
Year and year x compost rate interactions were apparent (P < 0.05) for K concentration and K removal, therefore data are reported by year. Forage K concentration followed a similar trend as P, by increasing (P < 0.001) linearly in each growing season (r2 = 0.27 and 0.44, respectively), ranging from 19.7 to 27.5 g kg1 in 20022003 and from 18.9 to 24.4 g kg1 in 20032004 (Table 2). Undersander and Naylor (1987) reported that Jose tall wheatgrass averaged 20.5 g K kg1, when harvested biweekly and 22.2 g K kg1 when harvested monthly, with values ranging from 16.2 to 25.0 g K kg1. In the present study, K concentration increased (P < 0.01) by 9 to 40% in 20022003 and 6 to 29% in 20032004 from the lowest to highest rate of compost application (Table 2).
The amount of K removed ranged from 96 to 262 kg K ha1 in 20022003 and 73 to 149 kg K ha1 in 20032004 (Table 2), which fit a curvilinear response curve (r2 = 0.76 and 0.58, respectively). The increase (P < 0.001) in K removal was due to greater yields during the 20022003 growing season. Several cool-season grasses have been reported to be luxury consumers of K (Cherney et al., 1998). Tall wheatgrass could also be considered a luxury consumer since K concentrations increased as soil K increased with compost rates despite adequate soil K in the untreated plots. Inorganic N fertilizer had no effect (P > 0.05) on K concentrations or the amount of K removed from the soil.
| CONCLUSIONS |
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| REFERENCES |
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