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Fig. 8. Dawn to dusk trends in mean leaf total nonstructural carbohydrates (TNC), consisting of water-soluble sugars (glucose, fructose, sucrose), low and high molecular weight fructans, and starch. Uppermost fully expanded sunlit spring wheat leaves were sampled for day after 50% emergence (DAE) and development stages given for 6 d during the 1993 (af) and 5 d during the 1994 growing seasons (gk) (note that during 1993 panels (e) and (f) are denoted by the right-most y axis, which is scaled half that of the left-most y axis). Symbols in legend, source of variance, and results from ANOVA are the same as described in Fig. 1. Each mean datum was derived from four individual assays (repeated measures) performed on a 30-mg sample (i.e., pooled sample taken from the thoroughly mixed powder of 10 freeze-dried leaves) across four replications at 2-h sample intervals (i.e., means based on n = 16). Vertical bars are 1 SE of replication means (i.e., n = 4). The above illustration was derived from measurements of as many as 9120 leaves.