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a USDA-ARS, Land Management and Water Conserv. Res. Unit, Pullman, WA 99164
b Skeen Hall N 140, New Mexico State Univ., Las Cruces, NM 88003
* Corresponding author (sharratt{at}wsu.edu)
Received for publication November 30, 2004.
| ABSTRACT |
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Abbreviations: LAI, leaf area index PAR, photosynthetically active radiation
| INTRODUCTION |
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Narrow-row corn (Zea mays L.) has been advocated in recent years as a technique to enhance grain yield (Orchard, 1998). Porter et al. (1997), for example, reported a 7% increase in grain yield in Minnesota while Nielsen (1988) found about a 3% higher grain yield in Indiana for corn grown in narrow rows (spacing less than 0.76 m) versus conventional rows (spacing of 0.76 m). More recently, Widdicombe and Thelen (2002) found that corn grown in narrow rows (spacing of 0.38 and 0.56 m) produced as much as 4% more grain compared with corn grown in conventional rows (spacing of 0.76 m) in Michigan. These differences in yield associated with row spacing appear to be accentuated for corn grown at more northerly locations within the U.S. Corn Belt. Paszkiewicz (1997), for example, found that corn grown in narrow rows to the north of Interstate 90 (44° N latitude) resulted in an 8% higher grain yield while that grown in narrow rows to the south of Interstate 90 resulted in a 4% higher grain yield compared with corn grown in wide conventional rows. Not all studies, however, have reported a positive response in yield to growing corn in narrower rows (Ottman and Welch, 1989; Westgate et al., 1997). In fact, Pedersen and Lauer (2003) found an 11% lower yield for corn grown in 0.19-m rows versus 0.38- and 0.76-m rows in Wisconsin while Farnham (2001) found a 2% lower yield for corn grown in 0.38-m rows versus 0.76-m rows in Iowa.
Hybrid and plant population may influence the yield response of corn to row spacing (Tollenaar, 1989). Farnham (2001) observed a significant hybrid x row spacing interaction among six hybrids grown in narrow and wide conventional rows in Iowa. Nielsen (1988) and Widdicombe and Thelen (2002), however, found that higher yields were attained for corn grown in narrow rows versus wide conventional rows irrespective of hybrids and plant populations tested in Indiana and Michigan.
Crop row spacing influences canopy architecture, which is a distinguishing characteristic that affects the utilization of light, water, and nutrients. Earlier canopy closure of corn grown in narrower rows has been found to enhance light interception (Ottman and Welch, 1989; Andrade et al., 2002) as well as suppress weed growth (Forcella et al., 1992). Westgate et al. (1997), however, reported that light interception was not affected by corn row spacing; they found no yield advantage to growing corn in narrow (spacing of 0.38 m) rows versus conventional (spacing of 0.76 m) rows over two growing seasons in Minnesota. Crop row spacing can also influence soil water utilization. Yao and Shaw (1964), for example, reported corn grown in 0.53-m rows used less water and used water more efficiently than that grown in 0.81- or 1.07-m rows. Karlen and Camp (1985) hypothesized that corn spaced more uniformly would reduce intrarow competition for water and thereby bolster yield.
Narrow-row corn has been advocated for enhancing grain production in corn due to less weed competition and better resource (soil water, solar radiation, and nutrients) utilization. Previous studies, however, have failed to adequately characterize the complexity of factors that bolster production of narrow-row corn. Therefore, the purpose of this study was to characterize root growth, water use, and microclimatic factors (e.g., soil temperature and evaporation) that may bolster grain production of corn grown in narrow rows versus wide conventional rows.
| MATERIALS AND METHODS |
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0.5% slope. Wheat (Triticum aestivum L.) was grown at the field site the year preceding the establishment of treatments in 1998 while corn was grown at the site the year preceding the 1999 growing season. The field site was cultivated with a chisel plow in autumn and with a disk to incorporate fertilizer at a rate of 170 kg N ha1, 40 kg P ha1, and 40 kg K ha1 in the spring before establishing the experimental treatments.
Agronomic Protocol
The experimental design was split plot with four replications. Row spacing was the main treatment and included corn sown in 0.38-, 0.57-, and 0.76-m rows. Corn hybrid was the secondary treatment and included Pioneer 3893 and DeKalb 417. Pioneer 3893 has a relative maturity of 90 d, is medium in stature, exhibits excellent early-season growth, tolerates drought, and has an upright, narrow-leaf structure. DeKalb 417 has a relative maturity of 91 d, is medium to tall in stature, exhibits excellent early-season growth, tolerates drought, and has a horizontal, wide-leaf structure. Individual plots were 9 by 15 m.
Corn was sown with a commercial corn planter at 150000 seeds ha1 in northsouth rows on 4 May 1998 and 19 May 1999. Weeds were controlled by hand or with an herbicide during the growing season. Plant stands were thinned by hand to 75000 plants ha1 shortly after emergence. Final plant population was determined at harvest on 17 Sept. 1998 and 27 Sept. 1999. Harvest consisted of removing ears from stalks by hand and then clipping the stalks at the soil surface from an area of 3.0, 4.6, and 6.1 m2 (equivalent to four adjacent crop rows, each 2 m long) within the 0.38-, 0.57-, and 0.76-m row-spacing treatments, respectively. The ear and stalk samples were dried at 60°C until constant weight, after which the ears were shelled to determine grain yield; residue biomass consisted of all remaining plant parts (stalks, leaves, husks, and cobs).
Root length density of Pioneer 3893 was measured on 20 July 1998 and 27 July 1999. These dates correspond to the silk or R1 developmental stage, which generally coincides with maximum root length density in corn (Durieux et al., 1994). Soil core samples (76-mm diam.) were extracted by machine to a depth of 1.5 m within and between rows at two locations in each plot. At each location, one core sample was taken midway between two adjacent corn rows, and two samples were taken between two adjacent plants within a crop row. The intrarow core samples were taken next to the stalk and midway between plants. Samples were sectioned to ascertain root length density at depth increments of 0.1 m for the 0- to 0.5-m depth and at depth increments of 0.2 m for the 0.5- to 1.5-m depth. The sectioned intrarow samples were consolidated into a single sample for each depth interval at each location. Root length density was determined by the line intersect method (Bohn, 1979). This method required soaking the soil samples in softened water and extracting the root material by sieving (nominal sieve openings of 1.0 and 0.5 mm). Root and other organic material retained by the sieves were placed in a glass dish filled with water. A grid was placed beneath the dish, and root length was then determined by counting the number of roots that intersected each grid line.
Microclimate
Instrumentation to measure crop water use, light interception, soil temperature, and soil evaporation was installed in each plot of Pioneer 3893 at the time of seedling emergence. Soil water content was assessed weekly in each plot by neutron attenuation and at the beginning and end of the season by gravimetric sampling. Soil water content was measured at 0.3-m depth increments to a depth of 2.1 m in the seed row and to a depth of 0.6 m between seed rows. Soil matric potential was measured using tensiometers placed at a depth of 1.75 and 2.0 m in one replication of each row-spacing treatment. These measurements, made weekly, aided in determining the direction and magnitude of water flow below the root zone.
Crop water use was calculated as the difference between precipitation plus soil water extraction and runoff. Water flow below the root zone was also considered in determining crop water use; downward flow signified drainage while upward flow contributed to evapotranspiration. Runoff was assumed negligible due to few intense rainfall events (two events in 1998 and 1999 that exceeded 40 mm d1), no visual rills or washing of debris at the soil surface immediately following these rainfall events (except on 14 July 1998 when washing of debris was apparent at the soil surface on all plots following a 49-mm precipitation event), and nearly level topography. Precipitation, soil water content, and water flow below the root zone were measured from emergence to harvest. Precipitation was measured daily at a nearby microclimate station (100 m from the experimental plots). Water flow below the root zone (WFBR) was determined according to:
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h is the difference in hydraulic potential (cm) over the depth interval
z (cm). Drainage occurred when water flow below the root zone was negative. Hydraulic conductivity was assumed to vary with soil water matric potential according to Campbell (1985):
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e is the air entry matric potential (cm),
is the matric potential (cm), and b is the slope of the natural log of the water retention curve. Sharratt and Gesch (2004) previously measured ks,
e, and b at the field site, but these parameters were measured at a depth of 1.0 to 1.25 m. Values of ks,
e, and b required for calculating water flow in this study (at a depth of 1.75 to 2.0 m) may differ from those previously measured due to changes in soil texture and bulk density (Campbell, 1985) with depth. Bulk density, but not texture, appears to increase with depth (from about 1.6 Mg m3 at 1 m to 1.7 Mg m3 at 2 m) based on pedon descriptions for Barnes loam near Morris, MN (USDA Natural Resources Conservation Service, 2004). Equations presented by Campbell (1985) suggest that the greater apparent bulk density at 2 m will reduce ks and
e by respectively 55 and 45% of the measured values at a depth of 1 m but will have little effect on b. These revised estimates of ks and
e were used to calculate hydraulic conductivity. Light interception was determined from incident PAR measured at the soil surface (Is) and above the crop canopy (Io). Intercepted PAR, calculated as Io Is, neglected PAR reflected from the canopy and soil surface (about 40 µmol m2 s1). The fraction of Io intercepted was calculated as (Io Is)/Io. Photosynthetically active radiation was simultaneously measured at the soil surface using a linear quantum sensor (LI-191SA, LI-COR, Inc., Lincoln, NE) and above the crop canopy using a quantum sensor (LI-190SB, LI-COR, Inc., Lincoln, NE). The linear quantum sensor was placed diagonally across one 0.56-m interrow, one 0.76-m interrow, or two adjacent 0.38-m interrows. Both ends of the sensor were positioned in the center of the crop row. Measurements were made at three locations in each plot within 1 h of solar noon on clear days: 27 May, 16 June, 24 June, 26 June, 1 July, 8 July, and 13 July 1998; 4 June, 14 June, 17 June, 24 June, 2 July, and 6 July 1999. Sensors were intercalibrated by measuring Io with both sensors after completing a series of measurements from a single replication. Measurements were initiated after seedling emergence and terminated near tasseling in 1998 and at about the V13 stage of development in 1999. Light interception was expressed as a function of thermal time from emergence where thermal time was computed using a minimum temperature threshold of 10°C and a maximum temperature threshold of 30°C (Swan et al., 1987).
Soil temperatures were measured at a depth of 10, 50, and 100 mm both within and between crop rows at three locations in each plot. Temperatures were measured using thermocouples; thermocouples at each depth were wired in parallel to obtain an average interrow and intrarow temperature. Thermocouples were monitored using a data logger, which sampled every 60 s and recorded hourly.
Soil evaporation was measured using microlysimeters similar to the design of Boast and Robertson (1982) with some modification. The microlysimeter consisted of two plastic pipes, one slightly larger (inside diameter, 92 mm; outside diameter, 101 mm) than the other (inside diameter, 81 mm; outside diameter, 89 mm) such that the smaller pipe fit inside the larger pipe. The larger pipe was 0.15 m long and reamed at one end to accommodate insertion of a 5-mm-thick 96-mm-diam. aluminum plate. The aluminum plate prevented moisture exchange but facilitated heat exchange between the soil inside and outside the microlysimeter. The larger pipe was semipermanently installed in the soil profile such that the aluminum cap made good contact with the subsoil and the top of the pipe was level with the soil surface. The smaller pipe was 0.15 m long and tapered at one end to facilitate insertion into the soil until the soil surface was level with the top of the pipe. The pipe was then excavated to extract an intact soil column. The bottom of the soil column was trimmed level with the end of the pipe. The outside of the pipe was cleaned, weighed, and then placed inside the larger-diameter pipe. The top of the smaller pipe protruded 5 mm above the soil surface, and the gap between the top of the smaller- and larger-diameter pipes was sealed with a rubber ring. The smaller-diameter pipe was reweighed every 24 h during an evaporation event (period of time with no precipitation). Soil inside the smaller-diameter pipe was discarded after 48 h or a rainfall event. Soil evaporation was measured midway between crop rows at three locations in each plot beginning 3 June, 22 June, 30 June, 8 July, and 13 July 1998 and 26 May, 14 June, 16 June, 21 June, 1 July, 6 July, 12 July, and 14 July 1999.
Homogeneity of sample variance was tested before analyzing agronomic data using a split-plot design and microclimatic data using a randomized block experimental design in analysis of variance. Least significant difference (LSD) was used to separate treatment effects when significant F values (P
0.10) were determined in the analysis of variance.
| RESULTS AND DISCUSSION |
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Agronomic Characteristics
Final plant population and ears per plant did not vary across row-spacing treatments in this study. Plant populations averaged 76300 and 75400 plants ha1 in 1998 and 1999, respectively, and ears per plant averaged 1.0 both years. Corn grain yield did not reflect differences in precipitation across years but varied from 10610 kg ha1 in 1998 to 9945 kg ha1 in 1999. Corn row spacing did not influence grain yield in 1998 (Table 1) but did affect yield in 1999 as corn grown in 0.38-m rows produced 10% more grain than corn grown in 0.76-m rows. This higher percentage in grain yield associated with narrower rows appears to be consistent with observations made by Paszkiewicz (1997), who found that corn grown in narrower rows resulted in an 8% higher grain yield at locations north of Interstate 90 in the USA. Although corn grown in narrower rows produced more grain in 1999, corn row spacing did not influence grain yield of Pioneer 3893 (probability of type I error or P = 0.38) or DeKalb 417 (P = 0.22) when averaged over both years of this study.
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The rooting depth of corn was observed to be about 0.9 m in this study (Fig. 1) and is consistent with other observations for corn in the north-central USA (Nickel et al., 1995), southeastern USA (Vepraskas et al., 1995), and Canada (Dwyer et al., 1988). Although not evident in Fig. 1, roots (root length density < 0.05 x 104 m m3) were detected below 0.9 m in 50% of the plots and below 1.1 m in 25% of the plots both years. Also apparent from Fig. 1 is that root length density was smaller in 1998 than in 1999. Averaged across treatments, root length density in the upper 0.5 m of the soil profile equaled 0.38 x 104 and 1.34 x 104 m m3 in 1998 and 1999, respectively. The greater root length density in 1999 may be attributed to greater seasonal precipitation. Wetter soils, however, could not account for the higher root length densities. In fact, at the time soil core samples were extracted from plots, water content in the upper 0.6 m of the soil profile averaged 0.37 and 0.29 m3 m3 across treatments in subsequent years. The soil was likely drier in 1999 as a result of less precipitation received before extracting samples in 1999 than in 1998. For example, 53 mm less precipitation was received within 7 d or 60 mm less precipitation was received within 14 d of sampling in 1999 than in 1998. Our finding of more prolific rooting in drier soil is consistent with recent observations by Merrill et al. (2002).
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The influence of corn row spacing on root length density was not consistent across years. Root length density differed among row-spacing treatments at some depth in both the interrow and intrarow positions in 1999 but only differed among treatments in the interrow position in 1998 (Fig. 1). Root length density in the interrow tended to be greater for corn grown in 0.38-m rows than in 0.76-m rows. Differences in root density in the interrow across years were found over a depth interval of 0.1 to 0.3 m in the soil profile. In contrast, root length density in the intrarow was greater for corn grown in 0.76- or 0.57-m rows than for corn grown in 0.36-m rows (Fig. 1). Differences in root density in the intrarow were found near the soil surface. The higher root density in the intrarow of 0.76-m rows is consistent with the closer spacing of plants within the 0.76-m rows than 0.36-m rows.
Microclimate
Differences in light interception among row-spacing treatments were observed during the 1998 and 1999 growing seasons (Fig. 2) . Light interception was typically greater for corn grown in 0.38-m rows than in 0.76-m rows with differences becoming apparent in early July when stems were rapidly elongating (V10 stage of development) and plants were more than 0.5 m in height. These differences in light interception among treatments, which persisted until tasseling in 1998, reflect those associated with leaf area or leaf architecture (i.e., distribution) within the canopy. Since leaf area or leaf distribution was not observed in this study, leaf area index (LAI) was estimated according to:
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is the light extinction coefficient for corn and was assumed to vary with row spacing according to Flenet et al. (1996). Estimates of LAI at the time light interception was measured both growing seasons indicated no differences (P > 0.1) in leaf area among row-spacing treatments. Scarsbrook and Doss (1973) also found that corn row spacing influenced light interception without necessarily affecting LAI, but their results varied with plant population and hybrid. We assume that differences in light interception among row-spacing treatments were associated with differences in leaf distribution within the canopy with a more uniform distribution of leaves in the canopy of narrow-row versus conventional-row corn.
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| CONCLUSIONS |
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| REFERENCES |
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