Published in Agron. J. 97:169-176 (2005).
© American Society of Agronomy
677 S. Segoe Rd., Madison, WI 53711 USA
Nutrient Uptake
Nitrogen Uptake across Site Specific Management Zones in Irrigated Corn Production Systems
D. Inmana,
R. Khoslaa,*,
D. G. Westfalla and
R. Reichb
a Dep. of Soil and Crop Sciences, Colorado State Univ., Fort Collins, CO 80523-1170
b Dep. of Forestry, Colorado State Univ., Fort Collins, CO 80523-1170
* Corresponding author (rkhosla{at}colostate.edu)
Received for publication March 25, 2004.
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ABSTRACT
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Development of improved fertilizer management practices has the potential to increase fertilizer use efficiency and improve environmental quality. The objectives of this study were (i) to characterize the within field spatial variability of N uptake across irrigated corn production fields, (ii) to quantify and compare N uptake and grain yield across three site specific management zones (SSMZs), and (iii) to compare grain yield response to applied N between management zones. This study was conducted on continuous corn (Zea mays L.) in irrigated fields in northeastern Colorado. Fields were classified into high, medium, and low site specific management zones. Treatments consisted of a control and two uniform N application rates over 2 site-years (one field over 2 consecutive yr and another field over 1 yr). Nitrogen fertilizer rates varied with site-year and ranged from 56 to 268 kg N ha1. Aboveground biomass samples were collected at physiological maturity and analyzed for total N. Between management zones, N uptake, grain yield, and grain yield response to applied N were found to be statistically different (p < 0.05). Management zones were found to be less spatially variable than the whole field. The SSMZs accurately characterized variability in N uptake as well as grain yield response to applied N. Thus, variation in N uptake and grain yield can potentially be managed using SSMZs.
Abbreviations: DGPS, differentially corrected global positioning system GIS, geographic information system GPS, global positioning system NUE, nitrogen use efficiency SSMZ, site specific management zone VRA, variable-rate application
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INTRODUCTION
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PLANT NUTRIENTS such as N, P, and K are often applied to plants to ensure economically viable grain yields in large-scale cropping systems (Swanson, 1982; Mengel, 1990). Development of improved nutrient management strategies has been a primary focus of agricultural research for over 10 yr (Gauer et al., 1992; Spellman et al., 1996; Mulla and Bhatti, 1997; Khosla and Alley, 1999; Khosla et al., 2002). Crop management strategies that improve nutrient use efficiency may increase farm profits and greatly reduce the deleterious environmental effects associated with fertilizer loss.
Nitrogen is often the most limiting nutrient in agro-ecosystems and is therefore applied in the highest quantities (Havlin et al., 1999; FAO, 2001). According to the Food and Agriculture Organization of the United Nations, about 82 million Mg of nitrogenous fertilizers were applied globally in 2001 (FAO, 2001). Of that, 60% was used for cereal production (Alexandratos, 1995). Raun and Johnson (1999) estimate worldwide nitrogen use efficiency (NUE) for cereal production to be 33%, meaning that in the year 2001 alone, approximately 33 million Mg of N fertilizer was lost. Although there are many causes and pathways for N loss, application of N in amounts that exceed crop requirements elevates postharvest NO3N levels in the soil and increases the potential for leaching of NO3N into groundwater supplies (Schepers et al., 1991).
In-field spatial variability of soil and crop parameters has long been recognized as affecting overall crop yield (Johnson et al., 2003). Studies indicate that within-field yield variation can be attributed to several factors including, but not limited to, variability in soil types, changes in landscape position, cropping history, soil physical and chemical properties, as well as nutrient availability across fields (Wibawa et al., 1993; Sawyer, 1994; Penney et al., 1996). Variable rate application (VRA) of N fertilizers to manage inherent soil spatial variability has been shown to increase NUE in some crops. In eastern Washington, Mulla and Bhatti (1997) showed that N application could be reduced by as much as 42 kg ha1 using VRA. Khosla and Alley (1999) found that using VRA on a 14.4-ha field in Virginia reduced total N applied by 22 kg N ha1 without a reduction in grain yield when compared with a uniform N treatment. Moreover, the results of Mulla and Bhatti (1997), Khosla and Alley (1999), Khosla et al. (2002), and Hornung et al. (2003) all demonstrated that N input optimization via high N application rates in more productive areas and low N rates in less productive areas has potential to increase NUE.
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Site Specific Management Zones
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The use of site specific management zones (SSMZ) for VRA has been shown to be a simple and effective way to increase NUE (Khosla et al., 2002; Hornung et al., 2003). Site specific management zones are defined as homogeneous subregions of a field that have similar yield limiting factors (Doerge, 1999; Khosla and Shaver, 2001). Numerous methods and combinations of methods have been used to delineate management zones including remotely sensed imagery (Bhatti et al., 1991), yield data (Mulla and Bhatti, 1997), farmer's experience combined with bare soil imagery and topography (Fleming et al., 1999), soil electrical conductivity (Sudduth et al., 1998), grid-soil sampling, and soil survey information (Franzen et al., 2000). Conceptually, using a management zone delineation technique, a production field could be classified into management zones that reflect the zone's productivity potential. For example, a field may be classified into three zoneshigh, medium, and low productivity potential management zones. Using the management zones approach, agricultural inputs are envisaged to be applied variably across the field in accordance with the productivity potential of the management zone. However, within a management zone, agricultural inputs are applied uniformly at a constant rate.
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Nitrogen Uptake and Variability
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Nitrogen management strategies that create a balance between N input and the crop N uptake could help alleviate problems associated with N loss (Grant et al., 2002). Accumulated soil N is highly susceptible to leaching and can potentially threaten groundwater supplies. Sustainable nutrient management practices should ideally replenish soil nutrients, which are depleted through crop uptake and harvest to soil fertility levels that can support economic crop growth and yield (Grant et al., 2002; Heckman et al., 2003). One means of maintaining soil N fertility levels without exceeding crop N requirements is to tailor N inputs to meet the specific crop N requirements. Published crop nutrient removal values are available from federal and state agencies (USDA, 2003) and have been used to help guide producers in making more informed application decisions. However, the usefulness of values reported in such publications is questionable. Heckman et al. (2003) made the point that "[nutrient removal] values that were established in the past may not be correct for current agronomic technologies such as hybrid, higher plant populations, yield potential, fertilizer practice, and soil conditions." In their study, Heckman et al. (2003) found that mean nutrient (N, P, and K) concentrations in corn (Zea mays L.) grain across 23 site-years were similar to published reference values. However, their results also revealed that nutrient concentration variability within a single corn hybrid grown across six site-years was as high as that of 10 different hybrids grown across 23 site-years (Heckman et al., 2003). Therefore, using average nutrient removal values as a means to estimate nutrient removal across varying conditions is questionable. For this reason most university soil analysis labs make N fertilizer recommendations based on a N requirement (amount of N required to produce one unit of grain yield) in conjunction with an estimated yield goal and residual soil NO3N test results.
Review of the current literature indicates that N uptake has not been thoroughly studied from a SSMZ perspective. We hypothesize that SSMZs can be used to effectively characterize N uptake spatial variability and could establish the usefulness for variable nutrient (N) application using SSMZs across the field.
The objectives of this study were (i) to characterize the within-field variability of N uptake across irrigated corn production fields, (ii) to quantify and compare N uptake and grain yield across three site specific management zones, and (iii) to compare grain yield response to applied N between site specific management zones.
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MATERIALS AND METHODS
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Study Sites
This study was conducted over 3 site-years (one field over 2 consecutive yr and another field over 1 yr). Sites were located in northeastern Colorado under a continuous corn cropping system, with center-pivot sprinkler irrigation for all site-years. Study sites ranged from 51 to 89 ha in size.
Site-years 1 and 2 were on a field mapped as having Bijou (coarse-loamy, mixed, superactive, mesic, Ustic Haplargids), Truckton (coarse-loamy, mixed, superactive, mesic, Aridic Argiustolls), and Valentine (mixed, mesic, Typic Ustipsamments) soil series (Soil Survey Staff, 1968). These soils are characterized as being very deep. The Truckton soil is well drained, Bijou is somewhat excessively drained, and Valentine is excessively drained. Both Truckton and Bijou soils are derived from arkose parent material and occur on terraces, fans, and uplands. Valentine soils are eolian derived and occur on uplands.
Site-year 3 was located on a field that was mapped as having Albinas (fine-loamy, mixed, superactive, mesic Pachic Argiustolls), Ascalon (fine-loamy, mixed, superactive, mesic, Aridic Argiustolls), and Haxton (fine-loamy, mixed, superactive, mesic Pachic Argiustolls) soil series (Soil Survey Staff, 1981). These soils are characterized as being very deep, well drained, and have accumulated carbonates in the soil solum. The Ascalon series occurs on upland positions and is formed from calcareous parent material. The Haxtun series consists of eolian deposits that overlay buried soil, occurring in drainages and depressions. The Albinas series is alluvial and occurs on fans and terraces.
Experimental Procedure
Before planting, soil samples were collected at depths of 0 to 30 and 30 to 60 cm. A systematic unaligned sampling grid was used with a sampling density of 2.5 samples per hectare on the entire field (independent of management zones). Each geo-referenced soil sample consisted of four to six cores that were composited into one sample. Soil samples were air-dried and ground to pass a 2-mm sieve (Soil Survey Staff, 1996). Soil pH was analyzed using a 1:1 soil/water mixture (Thomas, 1996). Particle size analysis was conducted using the hydrometer method (Bouyoucos, 1962). A summary of soil texture, pH, and organic matter for each site-year is presented in Table 1. Total NO3N was determined using the method of Mulvaney (1996). An interpolation technique, inverse-distance weighting, was used in ArcView (Version 3.2) to generate surfaces for soil texture, and soil organic matter.
Corn was planted at 75000 plants ha1 with a row spacing of 76 cm. Site-years 1 and 2 were planted with Pioneer hybrid 34G81 and site-year 3 was planted with Pioneer hybrid 34K77.
Site-specific management zones were delineated on all fields using the commercially available AgriTrak Professional software (Fleming et al., 1999). This program relies on three Geographic Information System (GIS) data layers: (i) bare soil aerial imagery on conventionally tilled land; (ii) farmer's perception of field topography; and (iii) farmer's past crop and soil management experience. These data layers were incorporated into a MapInfo (GIS) database to run mathematical interpolation surfaces to develop three management zones (Khosla et al., 2002). Traits such as dark color, low-lying topography, and historic high yields were designated as a zone of potentially high productivity or high zone. Details of this technique are provided in Fleming et al. (1999), Khosla et al. (2002), and Koch et al. (2004).
Nitrogen applications were made at the six-leaf crop growth stage (V6) using undiluted urea ammonium nitrate 32% solution applied with an eight-row cultivator. Nitrogen treatments were based on the N rate algorithm given by Mortvedt et al. (1996) for each site-year. The three N treatments were (i) the recommended N rate (as determined from the N rate algorithm), (ii) approximately half the recommended rate, and (iii) a control treatment (0 kg N ha1) (Table 2). Experimental strips were randomly allocated and consisted of 24 rows of corn that spanned the length of the field (>700 m). Treatments were replicated once and were nested within management zones. At the crop's physiological maturity (R6 growth stage) aboveground biomass samples were collected for grain yield and N content analysis. Four samples were randomly located and collected from each experimental unit (treatment in each management zone). Each sample consisted of two 1-m long sections of a corn row. Samples were separated into grain, husk and cob, and leaf and stalk portions; air-dried to a constant weight; analyzed for grain yield; and ground. Samples were then analyzed for total N concentration. Nitrogen uptake was found by the percentage of total N contained in the aboveground portion of the samples multiplied by the biomass weight and then converted to kg of N ha1. Grain yield was determined by converting the total grain weight per sample at 15.5% moisture to Mg ha1.
Fields, management zones, treatment strips, soil sample positions, and grain yield samples were all logged using a differentially corrected Trimble Ag 114 global positioning system (DGPS) unit. All GIS analysis and data processing were performed using MapInfo 7.0 and ArcView 3.2.
Data Analysis
Statistical analysis was performed using SPLUS 6.1 and SAS 8.0. Grain yield and N uptake differences between management zones were analyzed using a fixed-effect, two factor nested design analysis of variance (ANOVA) in which treatments were nested within management zones. In the ANOVA model, observational errors (i.e., subsamples within treatments) were nested within experimental errors. When ANOVA was found significant at P < 0.05, mean separation was performed using least squares difference (LSD) at P < 0.05. The spatial structure of the data was analyzed using semi-variogram plots. Moran's I was used to assess the spatial auto-correlation of N uptake and grain yield for each site-year and for each management zone within each site-year. A spatial auto-regressive model was used to analyze the spatial relationship between N uptake and grain yield within each site-year. Inverse distance weighting was used to create the spatial weight matrices used in Moran's I and spatial auto-regressive model. Since site-years 1 and 2 were located on the same field, an F test of unequal variance was used to test if grain yield and N uptake were equal across growing seasons at p < 0.05. Least squares regression analysis was used to model grain yield response to applied N and to examine the relationship between soil properties and N uptake. Best-fit models for grain yield response to N were determined using the All-Possible Regressions Procedure (SAS Inst., 2001). Indicator variables were introduced in the grain yield response model to test for differences among management zones (high, medium, and low). A complete review of the use and interpretation of indicator variables is provided by Neter et al. (1996).
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RESULTS AND DISCUSSION
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Within-Field Spatial Variability
Results from the semi-variograms indicate that both site-year 1 and 2 have significant spatial dependence for both N uptake and grain yield, with the former having stronger spatial structure (Fig. 1). Results from Moran's I and spatial auto-regressive model are presented in Table 3. Coefficients from Moran's I further support significant spatial dependence for both N uptake and grain yield. Mean N uptake, grain yield, and coefficient of variation (CV) for each site-year are shown in Table 4. Taylor et al. (1998) and Washmon (2002) used the CV as a measure of spatial variability for crop parameters. When the population of interest is spatially distributed, higher CVs indicate greater spatial variability. In our study, CV values for N uptake ranged from 22 to 35%; this coupled with the results of Moran's I indicates that N uptake had a high degree of spatial variability across the field. However, average N uptake values for all site-years were in agreement with published reference N uptake values (PPI, 2004). Average N uptake in this study ranged from 161 to 261 kg N ha1 across all site-years. Our results, like those of Heckman et al. (2003), raise questions about the usefulness of mean nutrient uptake values. Considering that the N uptake spatial variability was relatively high, using mean N uptake values to target N application rates potentially could have resulted in some portions of the fields being under- or over-fertilized.
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Table 3. Moran's I for whole field N uptake and grain yield and coefficient of determination from spatial auto-regressive models of N uptake and grain yield for all site-years.
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Because site-years 1 and 2 were on the same field over two consecutive growing seasons, we were interested in seeing if the spatial variability of N uptake was temporally stable. Site-years 1 and 2 had similar CVs for N uptake, 29 and 35%, respectively. An F test of unequal variances between site-years 1 and 2 indicated that variance in N uptake was statistically equal (p < 0.05) over the two growing seasons. In addition, for site-years 1 and 2 a negative linear relationship was found between sand percentage and N uptake as well as sand percentage and organic matter, r2 = 0.30, r2 = 0.51, p < 0.05, respectively. For the sites used in this study, regions of high sand content (8091% sand, Table 1) have less mineralizable N, less available water, and are more prone to N loss through leaching under irrigated systems and therefore lower crop N uptake. Results of the F test and regression analysis indicated that the similarity in spatial pattern variability across the field in site-years 1 and 2 is reflective of the spatial pattern of stable soil properties (i.e., texture).
Grain yield for all site-years was typical of this region (Table 4), with yields ranging from 9.9 to 11.6 Mg ha1. Grain yield variability was lower (CV = 1821%) than that observed for N uptake (CV = 2235%). Grain yield also exhibited less spatial variability than N uptake (Fig. 1, Table 3). A negative association was found between CV and grain yield. These results agree with those of Taylor et al. (1998) and Washmon (2002) in which an inverse relationship between CV and grain yield was reported. Higher overall yields are associated with yield uniformity and therefore low CVs (Taylor et al., 1998).
As with the N uptake results, CVs for grain yield between site-years 1 and 2 were similar, 20 and 21%, respectively (Table 4). Using an F test of unequal variances, it was found that grain yield variances were statistically equal between the two growing seasons (p < 0.05). Nitrogen uptake measurements taken at physiological maturity (R6 growth stage) were well correlated to grain yield in all site-years (r = 0.640.80, p < 0.05). These results were similar to those reported by Muchow (1988) and Katsvairo et al. (2003). The relationships were much stronger using spatial auto-regressive models (r2 = 0.970.98, p < 0.05), indicating that these two parameters are spatially dependant (Table 3). The outcome from the above statistical analyses (CVs, F test of unequal variances, and spatial autoregressive models) suggest that the patterns of spatial variability in soil properties correspond to patterns of spatial variability in grain yield. Our results indicate that the effectiveness of N management practices are limited by soil properties, for example, low yielding areas (low zone) may continue to be low yielding until the soil properties of such areas are improved. Therefore, the fields used in this study would be good candidates for site specific N management using a management zone approach. Fields that have highly variable crop parameters, due to variability in soil properties, would conceptually be better managed using the SSMZ approach.
Variability between Management Zones
Mean N uptake across SSMZs and N application treatments are presented in Fig. 2. The N uptake was found to be significantly different for the N application treatments in this study (P < 0.05). Mean N uptake increased with increasing SSMZ productivity potential. Nitrogen uptake differed significantly between SSMZs for all N application rates for site-years 1 and 2. This supports our hypothesis that SSMZs have the potential to characterize spatial variability in N uptake. Using the SSMZ approach, the low and high productivity zones were consistently separable for site-years 1 and 2 based on N uptake (p < 0.05).

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Fig. 2. Mean N uptake across low, medium, and high management zones for site-year 1, 2, and 3. Error bars represent standard deviation of the mean. Different letters are significantly different (p < 0.05).
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Medium productivity management zone separation was not as distinctive as separation of low and high management zones. Such a finding was not surprising because medium productivity zones contain isolated and small inclusions of both high and low management zones. Westfall et al. (2003) reported that a considerable amount of smoothing occurs in management zone delineation, making it feasible for commercial fertilizer application equipment to apply variable rate N within management zones.
Although not significantly different, site-year 3 mean N uptake values followed a similar trend as was observed for site-years 1 and 2. We believe N uptake values for site-year 3 were impacted by hail damage that occurred during the midvegetative crop growth stage (V-8 to V-10 crop growth stage). The damage was moderate, reducing the overall biomass and the crop's photosynthetic ability, and thus reducing overall crop N uptake across the field.
Mean grain yield across SSMZs and N application treatments are presented in Fig. 3. The grain yields were significantly different for the N application treatments in this study (P < 0.05). Overall, mean grain yield increased with increasing SSMZ productivity potential. In sites years 1 and 2, the medium zone was found to be statistically equal to the low zone for the control treatment and high zone for the 192 kg N ha1 treatment. Most likely, inclusions of isolated and small areas of low and high zones within the medium zone prevented an unambiguous classification of grain yield. For site-year 3, we observed a lack of response between management zones (p < 0.05) for the third N treatment (114 kg N ha1). However, the overall trend was similar to the trends seen in site-years 1 and 2.

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Fig. 3. Mean grain yield across low, medium, and high management zones for site-year 1, 2, and 3. Error bars represent one standard deviation of the mean. Different letters are significantly different (p < 0.05).
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Mean N uptake, average grain yield, CV, and Moran's I for SSMZs from each site-year are presented in Table 5. All N uptake CVs and Moran's I results (for the high, medium, and low management zones) were equal to or significantly lower than the CVs and Moran's I observed for the whole field. For site-years 1 and 2, CVs for mean grain yield were lower in the high and medium zones than the grain yield CVs observed for the whole field. For site-year 3, the medium and low zones had lower CVs for mean grain yield than the whole field. This was expected and logical because SSMZs are homogeneous subregions within a field (Doerge, 1999). Across SSMZs and site-years, N uptake and grain yield CVs ranged from 16 to 28% and 11 to 24%, respectively. Comparing the N uptake and grain yield CVs within management zones to CVs for the whole field, SSMZs were successful in differentiating spatial variability.
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Table 5. Mean N uptake, average grain yield, CV , and Moran's I for site-specific management zones (SSMZs) for all site-years.
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Nitrogen Response
Grain yield response to applied N for each site-year is presented in Fig. 4. Using regression analysis, the intercepts for SSMZs were statistically different (p < 0.05) for site-years 1 and 2. This indicates that each management zone may differ significantly in its capacity to utilize applied N. For site-years 1 and 2 we found that N response for SSMZs was best described using a curvilinear function, with asymptotes being reached at approximately 125 and 100 kg N ha1, respectively. Site-year 3 was modeled using a linear function. Intercepts were statistically different (p < 0.05) between the low and high and between the medium and high zones. Grain yield response to applied N for site-year 3 was most likely limited because of hail damage that occurred between the eight and 10-leaf crop growth stage.

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Fig. 4. Grain yield response to applied N across site-specific management zones in irrigated corn production systems for site-years 1, 2, and 3.
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CONCLUSIONS
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In this study, grain yield and N uptake across irrigated corn production fields was shown to exhibit significant spatial variability. The pattern and scale of the spatial variability was found to be stable over time. As anticipated, site specific management zones exhibited less N uptake and grain yield spatial variability within individual zones than on a whole field basis. Between management zones, N uptake and grain yield were statistically different. Grain yield response to N was also shown to be significantly different across management zones. This study showed that spatially variable crop parameters could potentially be managed using SSMZs. Furthermore, these results are encouraging because development of improved N application algorithms for site specific management must be consistent over time. More research is needed to develop site specific N recommendation algorithms that account for in-field N uptake variability.
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ACKNOWLEDGMENTS
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The authors acknowledge and thank the USDA-IFAFS and Agricultural Experiment Station for funding this research project. Our gratitude also goes out to Brad Koch, Andy Hornung, Chris Woodward, Tim Shaver, Bill Gangloff, and Kim Fleming for their support of this study.
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