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a Dep. of Anim. Sci., 327 Morrison Hall, Cornell Univ., Ithaca, NY 14853-4801
b Dep. of Crop and Soil Sci., Bradfield Hall, Cornell Univ., Ithaca, NY 14853-4801
* Corresponding author (djc6{at}cornell.edu)
Received for publication January 26, 2001.
| ABSTRACT |
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Abbreviations: DM, dry matter OM, organic matter
| INTRODUCTION |
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Concentrations of crop-available N in manure are inherently low and may be further reduced because of slow release of organically bound N and volatilization of NH3 from surface-applied manure (Beauchamp, 1983; Klausner and Guest, 1981). Manure applied on a regular basis, however, can be used to build up both soil organic matter (OM) and the N pool and provide a continuous source of nutrients for the crop. It can serve as an alternative in dry years when application of fertilizer N may not be economical. Nitrogen from both fertilizer and manure is susceptible to leaching and runoff as NO3; therefore, efficient management of manure and fertilizer N on cropland is important for improving the economics of crop production and minimizing adverse impacts on water quality (Jokela, 1992).
Several studies in the USA have focused on N recovery from manure by corn (Zea mays L.). These studies showed that corn has a low N recovery: 10 to 40% when 100 to 500 kg N ha-1 yr-1 was applied as manure (Klausner and Guest, 1981; Wiesler and Horst, 1993). Much of the cropland in the northeastern USA is poorly drained, which discourages the use of alfalfa (Medicago sativa L.). On these sites, grass provides a better option for N uptake from manure. Manure applications on alfalfa can adversely impact herbage production (Daliparthy et al., 1994; Lory, 1993), and manure applications to established stands are not recommended (Schmitt, 1998). Also, grasses have had a greater response to manure applications compared with alfalfa (Min et al., 1999). Researchers have pointed out the following advantages in using perennial grasses for nutrient uptake from manure: Grasses can utilize large quantities of nutrients, especially N; manure can be applied to perennial grasses several times throughout the growing season; grasslands pose less risk from leaching or runoff losses than cultivated fields; and there is less risk of pathogenic contamination of grasses than for edible crops (Bittman et al., 1999).
Apparent N recoveries of 20 to 50% were reported for perennial ryegrass (Lolium perenne L.)dominant grasslands receiving 200 to 450 kg N ha-1 yr-1 as manure (Davies, 1970). Kanneganti and Klausner (1994) demonstrated that intensively managed orchardgrass has the potential to absorb large quantities of manure N. On an annual basis, the orchardgrass crop recovered 430 kg ha-1 yr-1 soil N from plots receiving 150 kg ha-1 total N from liquid manure in combination with 600 kg ha-1 fertilizer N. Cool-season grasses often accumulate the majority of their annual DM production in the spring, and applications of manure in the spring should be most efficient at increasing DM yield. Schmitt et al. (1999b) determined that manure applied in the spring to reed canarygrass (Phalaris arundinacea L.) resulted in superior yields compared with split manure applications made after second and third harvests.
There have been few manure management studies in the USA involving perennial grasses. To optimize manure management practices in the region, more information is needed on the yield response of perennial grasses to manure applications and the subsequent removal of N. Orchardgrass is well adapted to the northeastern USA, with the potential for high yields and relatively high N recovery from applied manure (Kanneganti and Klausner, 1994). Tall fescue has the potential for greater yields than orchardgrass, and tall fescue stands have persisted better than orchardgrass under high manure applications (Min et al., 1999). Our objective was to compare yield and N utilization by orchardgrass and tall fescue from dairy manure or commercial fertilizer N sources.
| MATERIALS AND METHODS |
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Semisolid dairy manure (Table 2) was split-applied each year (19941997) at spring green-up and after first harvest. The common range in seasonal dairy manure application is 22.4 to 67.2 Mg ha-1 fresh wt. (Magdoff and van Es, 2000), with the high manure rate in this experiment set at the top of the common range. Manure application on forage crops must not result in any contamination of harvested forage, and the maximum amount of manure that can be applied with minimum risk of contamination primarily depends on the physical form of the manure. Based on previous experience, we estimated that at least 34 Mg ha-1 dry matter (DM) of semisolid manure could be used in a single application with minimal risk of contaminating harvested forage. Manure came from a dairy farm that used sawdust bedding, resulting in a consistent product that could be weighed easily and distributed uniformly by manual techniques.
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Nitrogen fertilizer was applied in lieu of manure in the spring of 1994 because plants were not yet well established. Rainfall amount and distribution during the 1994 growing season were favorable enough to allow four harvests. Three harvests were taken during the 1995 to 1997 growing seasons. Manure applications were terminated after 1997, and N fertilizer was applied to all previous treatments from 1998 to 2000 (Table 3). Residual effects of manure applications under a recommended N fertilization regime were assessed. A two-harvest management was used from 1998 to 2000 as appropriate for nonlactating dairy cow forage (Cherney and Cherney, 2000).
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Yields were determined by harvesting a 1- by 6.1-m strip of each plot using a flail mower. Clipping height was 0.1 m. Four harvests were taken in 1994, three harvests each year during 1995 to 1997, and two harvests each during 1998 to 2000. Whole-plant samples were collected, dried at 60°C for 72 h, and ground in a cyclone mill (Udy Corp., Fort Collins, CO) to pass through a 1-mm screen. Concentrations of N in samples collected from 1994 to 1997 were determined by Kjeldahl procedure (AOAC, 1990). Samples collected from 1998 to 2000 were analyzed for N by Dumas dry combustion with thermal conductivity detection using a LECO analyzer (Model FP-528, LECO Corp., St. Joseph, MI). Dumas combustion with thermal conductivity detection has been shown to have precision and accuracy at least as good as Kjeldahl methods, with similar N values (Tate, 1994). Concentrations were expressed on a DM basis.
Eight soil cores were collected and composited from each subplot (0.20-m depth) immediately following fall harvest from 1997 to 2000. Soil samples were air-dried, manually crushed, and passed through a 2-mm sieve. Soil pH (1:1 soil/water suspension) was measured (McLean, 1982). Soil OM was determined by loss on ignition, Method S-1811 of Cornell Nutrient Analysis Laboratory (McClenahan and Ferguson, 1989).
Statistical significance of treatment effects and interactions was determined using PROC MIXED (Littell et al., 1996) in SAS version 7.0 software (SAS Inst., 1998) for a split-plot analysis of variance with repeated measures. Forage yield, tissue N, and N removal were analyzed by three sets of years (1994, 19951997, and 19982000) due to different manure and fertilizer treatments (Table 2). Soil data were analyzed by plant species and sets of years (1997 and 19992000). The models assumed that nutrient treatments, plant species, and harvest (when applicable) were fixed variables while replication was a random variable. Means of nutrient treatments were separated by plant species and harvest within a year by the TukeyKramer procedure for multiple comparisons (P
0.05).
| RESULTS AND DISCUSSION |
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Yield
Stand persistence of the species in our study was unaffected by nutrient treatments, with essentially pure stands of orchardgrass and tall fescue through the 2000 harvest season. At least 95% of each individual plot was estimated to be composed of the seeded grass species, using visual estimation of plots in the spring of 2000 (data not shown). In Maryland, stand ratings of orchardgrass fertilized with manure at several application rates were not affected by harvest management (Min and Vough, 2000) although thinning of orchardgrass stands fertilized with high levels of N has been reported (Washko et al., 1967). Declines in estimated stand persistence of perennial grasses have been documented in other studies when untreated checks were compared with N fertilizer or manure treatments (Decker et al., 1967; Schmitt et al., 1999b).
1994
Annual yield was not different among nutrient treatments in 1994 (P > 0.05), primarily because all nutrient treatments received the same N fertilizer application in the spring of 1994. Yields of harvests within the season differed (P
0.01); most of the forage production occurred in the spring, with low yields in the fall. Mean yield proportions for Harvests 1, 2, 3, and 4 in 1994 were 0.48, 0.15, 0.28, and 0.09, respectively. Tall fescue consistently produced higher yields than orchardgrass at all harvests (Table 4; P
0.05), with tall fescue yields as high as 13.8 Mg ha-1 for NT-1. Tall fescue also outyielded orchardgrass in a Maryland study when rainfall was abundant (Min et al., 1999). Nutrient treatment x harvest and species x harvest interactions were significant (P
0.05) in 1994. While tall fescue produced 28% more DM in the spring compared with orchardgrass, it produced 51 and 131% more DM at the second and fourth harvests, respectively, compared with orchardgrass. Nutrient Treatment 1 had numerically greater yields than NT-3 for the first two harvests, but NT-3 yields exceeded those of NT-1 for the last two harvests, resulting in a nutrient treatment x harvest interaction (P
0.01).
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0.01) each year, with orchardgrass averaging 26.7% more yield than tall fescue for the spring harvest while fescue yielded more DM for both regrowth harvests each year (Fig. 1)
. Although varieties were selected to have similar heading dates, even a late-maturing orchardgrass variety apparently accumulates DM earlier in the spring than tall fescue. In 1995, May-through-August rainfall was 51% of what occurred on average in 1996 and 1997. This resulted in most of the annual DM in 1995 being produced in the spring. More rainfall and better-distributed rainfall in 1996 and 1997 resulted in a DM distribution of 27.4, 39.9, and 32.7% for Harvests 1, 2, and 3, respectively, of tall fescue (Fig. 1). In a Minnesota study, with near-normal seasonal rainfall and 200 kg N ha-1, only 30% of the annual DM production of reed canarygrass was taken in the first harvest, with 57% in the second harvest and 12% in the third harvest (Vetsch et al., 1999).
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0.01). Dry matter yields from NT-1 were much higher than from the manure treatments in 1995 (Table 4 means averaged over species) but were not consistently higher than those of NT-3 after the 1995 growing season. In 1996, NT-1 produced greater DM yields than NT-3 at the spring harvest but yielded less DM than NT-3 for regrowth harvests, resulting in a nutrient treatment x harvest interaction (P
0.01). In 1997, NT-1 yielded less DM than NT-3 at the spring harvest but yielded greater DM at regrowth harvests, also resulting in a nutrient treatment x harvest interaction (P
0.01). Other two-factor interaction effects on seasonal yield were significant in 1995 to 1997 (P
0.05) but were caused by differences in magnitude of response not direction of response. A three-harvest management system under low rainfall in 1995 resulted in yields averaging 37% of 1994 yields. A three-harvest management system typically results in about 15% higher yield than a four-harvest management system in New York when harvest managements are compared under the same environment in the same year (J.H. Cherney, unpublished data, 1997). After 2 yr of manure applications, DM yields from NT-3 in 1996 were statistically the same (P > 0.05) as those from the NT-1, presumably as a result of manure nutrient buildup (Table 4). Nutrient Treatments 1 and 3 also did not differ (P > 0.05) in total DM yield in 1997. The lower manure rate (NT-2) predictably resulted in the lowest DM yields throughout the experiment.
1998 to 2000
From 1998 to 2000, no manure was applied, and all nutrient treatments received 168 kg N ha-1 to assess any residual effects of manure applications beyond recommended N fertilizer application. The previous manure treatments (NT-2 and NT-3) yielded greater DM (P
0.01) than NT-1 (Table 4). From 1998 to 2000, NT-2 averaged 29% greater DM yield than NT-1 while NT-3 averaged 44% greater yield that NT-1. From 1998 to 2000, orchardgrass averaged 51% greater DM yield in manure treatments than in NT-1 while tall fescue averaged 24% greater DM yield with manure treatments. Residual effects on yield from manure treatments were likely a combination of additional N release due to manure along with other beneficial effects. In Vermont, manure applications to continuous corn for 11 yr resulted in beneficial biological, physical, and chemical changes to the soil (Magdoff and Amadon, 1980). Residual yield differences may have been the result of residual positive effects from manure treatments combined with residual negative effects from relatively high application rates of commercial N fertilizer on NT-1. In a separate study, after 3 yr of three rates of commercial N fertilizer application to tall fescue, all plots were fertilized at the same N rate in the fourth production year, and check plots produced 35% higher yields than plots fertilized with 224 kg ha-1 N fertilizer for the three previous years (J.H. Cherney, unpublished, 1999).
As occurred from 1995 to 1997, a species x harvest interaction (P
0.01) was the result of greater orchardgrass yields for spring harvest with lower yields for the regrowth harvest compared with tall fescue for 1998 to 2000 (Fig. 2)
. A less intensive two-harvest management with a delayed spring harvest starting in 1998 allowed tall fescue more opportunity to catch up to the early season DM production of orchardgrass. While spring harvest orchardgrass yields averaged 26.7% greater than those of tall fescue under a three-harvest regime, orchardgrass averaged only 8.9% greater yields than fescue under a two-harvest regime from 1998 to 2000.
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Nitrogen
Forage Nitrogen
Concentrations of N in forage from manure treatments were more variable than those from N fertilizer (data not shown). Species x harvest and nutrient treatment x harvest interactions in 1994 (P
0.05) were due to differences in magnitude, not direction of response, but also were confounded by the fact that all nutrient treatments received the same N fertilizer application at spring green-up in 1994. Fall harvest N concentrations in this study were not consistently lower than N concentrations in spring and summer harvests, averaging 18.5 g kg-1 for Harvest 1, 16.4 for Harvest 2, and 17.7 for Harvest 3 during 1995 to 1997. This is consistent with studies conducted with reed canarygrass grown under optimum N fertilization (Vetsch et al., 1999). From 1995 to 1997, there were species x harvest interaction effects (P
0.01) on forage N concentration that followed the same pattern, with similar species N concentrations in the spring harvest and much greater N concentrations in orchardgrass compared with fescue at regrowth harvests. Orchardgrass averaged 19.9, 18.5, and 20.4 g N kg-1 DM in Harvests 1 to 3, respectively, while fescue averaged 18.5, 14.3, and 15.1 g N kg-1 DM in Harvests 1 to 3 respectively, from 1995 to 1997. Nutrient treatment x harvest interaction effects (P
0.05) on N concentration from 1995 to 1997 generally were the result of greater N concentrations due to NT-1 compared with manure treatments at spring harvest. In 1996 and 1997, N concentrations in NT-3 were similar to those in NT-1 at regrowth harvests (data not shown).
Species x harvest interactions (P
0.05) from 1998 to 2000 were due to differences in magnitude of response. Across all nutrient treatments and species, N concentration was higher in fall regrowth than in spring growth under a two-harvest system, averaging 16.4 and 22.5 g kg-1 for Harvest 1 and 2, respectively. Orchardgrass was consistently greater in N concentration, averaging 16.9 and 25.4 g N kg-1 DM at spring harvest and at the regrowth harvest, respectively, compared with 15.9 and 19.7 g N kg-1 DM at spring harvest and at regrowth, respectively, of fescue from 1998 to 2000. In 1998, a nutrient treatment x harvest interaction (P
0.01) was the result of similar N concentrations among nutrient treatments at spring harvest but greater N concentration due to NT-3 in regrowth compared with the other nutrient treatments. In 2000, a nutrient treatment x harvest interaction (P < 0.01) was due to greater N concentration in NT-1 compared with the other treatments at spring harvest, with similar N concentrations among nutrient treatments in regrowth.
Nitrogen Removal
In 1994, tall fescue removed 241 kg N ha-1, compared with 205 kg N ha-1 in orchardgrass (P < 0.01; Table 4), due to the much greater DM yield of fescue. Nutrient Treatment 1 removed 251 kg N ha-1, which was greater than 207 and 211 kg N ha-1 from NT-2 and NT-3, respectively. From 1995 to 1997, N removal by the two species was similar (P > 0.05). A nutrient treatment x year interaction effect (P
0.01) on N removal from 1995 to 1997 resulted from much greater N removal from NT-1 than from other nutrient treatments in 1995 but no difference in N removal between NT-1 and NT-3 in 1996 or 1997. A nutrient treatment x year interaction from 1998 to 2000 was due to differences in magnitude. All nutrient treatments were different (P
0.05) from 1998 to 2000, removing 124, 153, and 178 kg N ha-1 from NT-1, NT-2, and NT-3, respectively (Table 4). A species x year interaction (P < 0.05) from 1998 to 2000 resulted from fescue removing more N than orchardgrass in 1998 and removing less than orchardgrass in 1999 and 2000.
Nitrogen Recovery
Relative N recovery was estimated for each year during the 1995 to 1997 period by calculating N removal each year as a proportion of total N applied during that year (Table 5). These relative recoveries, however, do not subtract N supplied from soil when no manure or fertilizer N was applied. Therefore, these calculations are overestimates of actual recovery of applied N. Relative N recovery tended to be lower in the manure treatments than in NT-1, especially at the higher manure rate (Table 5). Relative N recovery between manure treatments was different (P
0.05) only in orchardgrass in 1995. Relative N recovery was very consistent in 1996 and 1997 with near-normal growing season precipitation. Below-normal precipitation during the 1995 growing season reduced yields, resulting in reduced relative N recovery compared with 1996 and 1997. In the year following a drought, N management of grass stands that received previous manure applications should take into consideration an increased level of N carryover.
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0.01) during 1999 and 2000, with greater soil OM in NT-1 compared with manure treatments for orchardgrass but less soil OM in NT-1 compared with manure treatments for fescue. Soil NO3 concentration in the top 0 to 20 cm of soil was at or below initial values by 2000 (data not shown), but it is possible that NO3 may have leached lower in the soil profile (Vetsch et al., 1999). | CONCLUSIONS |
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| ACKNOWLEDGMENTS |
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| REFERENCES |
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