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a Dep. of Agron. and Plant Genetics, Univ. of Minnesota, 1991 Upper Buford Circle, St. Paul, MN 55108
b USDA-ARS, Weed Sci. Lab., BARC, 10300 Baltimore Blvd., Beltsville, MD 20705
Corresponding author (sheaf001{at}maroon.tc.umn.edu)
| ABSTRACT |
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8.8 Mg ha-1). Because adapted grain soybean was more mature and had a greater pod proportion than forage soybean, grain soybean had greater crude protein (CP) and lower fiber concentration than forage soybean. Average forage CP for forage and grain types was 146 and 218 g kg-1, respectively, while neutral-detergent fiber (NDF) concentration was 523 and 400 g kg-1, respectively. Decreasing row width from 76 to 25 cm increased forage yield 0.8 Mg ha-1 but had no effect on total herbage quality.
Abbreviations: ADF, acid-detergent fiber ANOVA, analysis of variance CP, crude protein NDF, neutral-detergent fiber NIRS, near-infrared reflectance spectroscopy WISC.BK, Wisconsin Black soybean
| INTRODUCTION |
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Limited research on use of soybean for forage shows the potential benefits of soybean as a forage crop. Hintz et al. (1992) reported forage yields from grain-type soybean in Wisconsin ranging from 2.4 to 7.4 Mg ha-1, depending on the stage of maturity at harvest. They concluded that grain soybean cultivars harvested at the R7 stage (one seed pod at mature color; 50% of leaves yellow) produced forage that was similar in quality to alfalfa harvested at early bloom. When soybean was grown as an intercrop with corn, the forage CP concentration was increased by 30 to 43% compared with corn alone (Herbert et al., 1984). Forage yields of the soybean and corn intercrop were comparable with those of the corn monoculture. Sod-seeding soybean into tall fescue (Festuca arundinacea Schreb) increased forage yield by 300% compared with tall fescue alone and increased the CP concentration of the forage by 10% (Ocumpaugh et al., 1981).
As soybean matures from stage R1 (beginning bloom) to R7, the leaf proportion declines (Fehr et al., 1971). Changes in the stem proportion with soybean maturation are less consistent. Hintz and Albrecht (1994) reported that the leaf concentration of grain soybean decreased from 708 g kg-1 at R1 to 168 g kg-1 at R7. Meanwhile, the stem fraction increased from 292 g kg-1 at R1 to 383 g kg-1 at R5 (beginning seed development) and then declined to 283 g kg-1 at R7 as the pod and seed components increased.
The harvest of soybean for forage at R6 (full seed) to R7 maximizes both the dry matter yield and forage quality (Hintz et al., 1992; Munoz et al., 1983). However, while the dry matter yield of soybean forage typically increases with advancing maturity, changes in the forage quality are less consistent. This is due to changes in the proportions of the leaf, stem, and pod fractions as well as the translocation of nutrients to the grain and increases in the lipid concentration of the seed (Hanway and Weber, 1971). Hintz et al. (1992) reported that CP concentrations declined from R1 to R3, remained constant between R3 and R5, and increased from R5 to R7. Whole-plant fiber concentrations increased from R1 to R5 and decreased from R5 to R7.
Reducing row spacing from a traditional width of 76 cm to 25 cm or less is recommended to enhance soybean grain yields (Naeve, 1999). In southern Minnesota, grain yields are typically increased by about 5%. Likewise, Hintz et al. (1992) reported that for grain soybean, a 20-cm row spacing produced more forage than a 76-cm row spacing. They also observed that decreasing row spacing increased the stem diameter but that row spacing had no effect on the total forage quality.
Tall forage soybean cultivars in maturity groups V, VI, and VII that reach a height of 1.5 to 2 m and have high yield potential have recently been released (Devine and Hatley, 1998; Devine et al., 1998a, 1998b). The forage soybean cultivars `Derry', `Donegal', and `Tyrone' had average forage yields of 9.3 Mg ha-1 compared with 7.6 Mg ha-1 for grain soybean cultivars in Iowa, New York, and Virginia. These new forage soybean cultivars have different relative maturity and stem traits than grain cultivars and may respond differently to changes in row spacing and harvest date. Our objective was to determine the effect of the harvest date and row spacing on the forage yield and quality of forage soybean in the upper Midwest.
| Materials and methods |
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The 1995 experiment was planted on 5 May at Rosemount and 2 May at Waseca. The experimental design was a split-plot with three replicates. Whole plots were two harvest dates and subplots were 10 soybean entries. All of the plots were 3 m wide by 6 m long, with soybean planted at 90 kg ha-1 (475000 seeds ha-1). The plots contained 4 rows spaced 76 cm apart. The soybean entries planted in 1995 included seven forage types: The varieties Tyrone, Derry, and Donegal (Devine and Hatley, 1998; Devine et al., 1998a, 1998b) and the experimental lines OR 13-12-3, OR 14-11-2, OR 19-12-2, and PA 5-2-1 that were developed at Beltsville, MD. Two types of standard grain soybean was included: `Sturdy', developed at the University of Minnesota as a high-yielding grain soybean (Orf et al., 1991); and `IA 2008 BC', developed at Iowa State University. Wisconsin Black (WISC.BK; PI 153271), an older forage-type soybean of Belgian origin, was also included (USDA-ARS, 1999). The maturity groups for soybean entries are shown in Table 1.
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Each year, the soybean forage yield was measured by harvesting a 2- by 5- m area from the middle of each plot. A randomly collected 10-plant subsample of soybean forage was manually cut to a 2-cm height and was chopped for analysis of the whole-plant moisture and forage quality. The whole-plant moisture content was determined by drying a 500-g sample at 60°C for 48 h. An additional 10-plant subsample was staged for maturity (Fehr et al., 1971) and separated into leaf, stem, and pod fractions before drying. The leaf fraction included leaves and petioles, the stem fraction included stems, and the pod fraction included pods and seeds.
Soybean forage was harvested on 31 Aug. and 19 Sept. 1995 at Rosemount and on 8 and 22 Sept. 1995 at Waseca. All of the entries were harvested at the early harvest date at both locations. Only the seven forage types were harvested at the late harvest date in 1995 because the early maturing grain types and WISC.BK had significant leaf loss. In 1996, the seven soybean entries were harvested on 3 and 19 September at Rosemount and on 10 September and 1 October at Waseca. The early and late harvest dates were selected based on an average target maturity stages of R6 and R7 for the grain soybean, respectively. Because of the diversity of maturity among the entries, it was not possible to harvest all of the entries at a similar maturity.
In 1995, forage quality analysis was conducted on OR 13-12-3, OR 14-11-2, PA 5-2-1, Sturdy, and WISC.BK at the early harvest and on OR 13-12-3, OR 14-11-2, and PA 5-2-1 at the late harvest. In 1996, forage quality analysis was done on Derry, PA 5-2-1, Tyrone, and Sturdy at both harvests. Whole-plant and plant fraction samples were ground to pass a 1-mm screen. Forage dry matter subsamples of whole-plant samples and plant components of selected entries were analyzed for CP, acid-detergent fiber (ADF), and NDF via near-infrared reflectance spectroscopy (NIRS). Spectra for NIRS analysis were collected with a NIRSystems1 (Silver Springs, MD) Model 6500 scanning monochrometer with a range of 400 to 2500 nm. A calibration set of samples was selected. Samples in the calibration set were analyzed for NDF and ADF according to the procedures of Goering and Van Soest (1970) and for Kjeldahl CP (Kjeldahl N x 6.25). Results from analysis of the calibration set were used to develop prediction equations using the Infrasoft International (ISI, Port Matilda, PA) NIRS 3 v. 4.0 software program Calibrate, with the modified partial least squares regression option (Shenk and Westerhaus, 1991). The standard errors of calibration for CP, ADF, and NDF were 8.0, 9.4, and 8.3 g kg-1, respectively, and the R2 values for all variables were >0.99.
Statistical Analysis
Data on the yield; proportion of leaf, stem, and pod components; and forage quality of the leaf, stem, pod, and total herbage were subjected to analyses of variance via the general linear models (GLM) procedure of SAS (SAS Inst., 1996). Years were analyzed separately because different soybean entries and different experimental designs were used in 1995 and 1996. In 1995, data on the forage yield and the leaf, stem, and pod proportion for the seven forage soybean were analyzed as a split-plot design, with the harvest dates as whole plots and the entries as subplots. The homogeneity of error variances was tested on the within-location analyses using Bartlett's test (Steel and Torrie, 1980). The combined analysis across two locations was done following the procedures of Gomez and Gomez (1984). Data on the forage yield and the leaf, stem, and pod proportion for 10 soybean entries in the early harvest were analyzed in a split-plot design, with the entries as treatments and combined across two locations. Forage quality data for five soybean entries at early harvest were analyzed in a split-plot design, with the entries as treatments and combined across two locations. The randomized complete block analysis showed significant location x entry interactions for forage quality, so three contrasts of entries were tested within the locations: grain type vs. forage type, grain type vs. WISC.BK, and forage type vs. WISC.BK.
In 1996, data on the forage yield and the leaf, stem, and pod proportion from seven entries, including five tall forage types and two grain types, were analyzed as a split split-plot design, with the harvest dates as whole plots, the row widths as subplots, and the soybean entries as sub-subplots combined across two locations. A similar split split-plot design combined across locations was used to analyze the forage quality data from Derry, PA 5-2-1, Tyrone, and Sturdy. There were significant location x harvest date x entry interactions for the total herbage CP, ADF, and NDF; however, the entry rankings remained the same in all four location x harvest date combinations, and there were only magnitude differences between the harvests. Therefore, a grain type vs. forage type contrast was tested within locations following the split split-plot analyses.
| Results and discussion |
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The soybean entries differed in yield, but the differences were not consistent over years and locations within forage or grain types. The soybean type and maturity group were confounded in this study because the forage-type soybean entries were in maturity groups V, VI, or VII, but the grain entries and WISC.BK were in maturity groups I or II. Contrast analysis revealed that there were yield differences between the soybean types at Rosemount and Waseca in 1995 and at Waseca in 1996 (Table 2). The forage and grain-type soybean yielded 25 to 50% more than WISC.BK in 1995 (Table 3). Grain and forage types had similar average yields at Rosemount in both years, but the forage types had average yields that were slightly higher at Waseca. These small differences in yield between forage and grain types contrast to the 23% yield advantage reported in Iowa (Devine et al., 1998a) for the forage type, Derry, compared with a standard grain soybean, `Sherman'. A comparison of the average 1995 grain-type yields from the early harvest with the average forage-type yields from the late harvest showed greater yields from the forage types at both locations (Fig. 1)
even though the late-harvested forage types still lagged in maturity compared with the early-harvested grain types. The grain and forage soybean yields were similar at the early and late harvests at both locations in 1996 even though the forage types lagged an average of three R stages behind the grain types at both harvests. These results suggest that forage soybean would have superior dry matter yields to the grain types if harvested at a similar maturity stage. Our maximum forage yields (
9 and 10 Mg ha-1 for grain and forage soybean, respectively) exceeded those reported by Hintz et al. (1992) for grain soybean in southern Wisconsin. Our soybean forage yields were often greater than those reported for commonly used single- or multiple-cut alternative forages grown in Minnesota such as sudangrass [Sorghum x drummondii (Steudel) Millsp. & Chase] and pearl millet [Pennisteum americanum (L.) Leeke] (Martin and Linn, 1992).
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Within a harvest date, forage soybean had more leaf and stem yield and less pod yield than grain soybean each year at both locations and more leaf and stem yield and less pod yield than WISC.BK at the early harvest in 1995 (Fig. 1). A comparison of soybean types for the leaf, stem, and pod proportion was possible at the early harvest in 1995. The dry matter yield of forage soybean at an average maturity of R3 was composed of approximately equal proportions of leaf and stem material, with very little yield from pods. The more mature (R6 and R7) grain entries and WISC.BK contained 440 and 580 g kg-1 pods at Rosemount and Waseca, respectively. A comparison of forage and grain soybean in 1996 showed that the pod contribution to the forage yield of forage soybean was minimal at the early harvest and 10 g kg-1 at the late harvest. The leaf contribution was 400 g kg-1 for forage types at the late harvest. The pod contribution for the grain soybean increased from 350 g kg-1 at the early harvest to 530 g kg-1 at the late harvest at Rosemount and from 450 to 660 g kg-1 at Waseca. Increases in the grain-type pod proportion were accompanied by decreases in the leaf proportion and an increase in maturityfrom R6 to R7. Hintz and Albrecht (1994) reported a leaf, stem, and pod proportion of 168, 282, and 485 g kg-1, respectively, for grain soybean at R7. Thus, the grain soybean and WISC.BK in this study had leaf, stem, and pod proportions that were similar to previous reports; but the less-mature forage soybean entries had less pod and more leaves.
Maturity and possibly other differences between soybean types affected the forage quality of the leaves, stems, and pods. As previously reported by Hintz et al. (1992) for grain soybean, the stems of all of the soybean entries had a lower forage quality; that is, they had lower CP and a higher ADF and NDF than the leaves or pods. In our study, the leaves of forage soybean usually had higher CP than the leaves of grain soybean because forage soybean had no significant seed production (Fig. 2 and 3) . During grain formation, N is translocated from the leaves to grain (Hanway and Weber, 1971). Grain and WISC.BK soybean had pods that were higher in quality than leaves, but for forage types with little seed development, the pods often had a forage quality that was similar to leaves. The lower pod CP and higher pod ADF and NDF in forage than in grain soybean was likely due to a greater proportion of high-quality seeds in the more mature grain types. By harvest at Rosemount in 1995, and at both locations in 1996, the only forage soybean with pod development was PA 5-2-1; thus, the values for the pod quality at these sites are averages for this entry.
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. There was no row width x entry interaction, which indicated that narrow rows increased yields similarly for both forage and grain soybean. Hintz et al. (1992) had reported a 1.2 Mg ha-1 yield increase for grain soybean as the row spacing decreased from 76 to 20 cm.
The forage quality of the total herbage, leaves, and pods was not affected by the row width, but the row width had a small effect on the stem ADF and NDF concentration. The stem ADF concentration was 596 and 603 g kg-1, respectively, for narrow and wide rows
. The stem NDF concentration was 707 and 719 g kg-1, respectively, for narrow and wide rows
. These forage quality differences are not likely to have practical significance, especially because differences in the total herbage forage quality were apparently determined mostly by the pod fraction. Our results on the impact of row spacing on yield and quality are consistent with those of Hintz et al. (1992), except we saw no effect of the row spacing on the CP concentration, and our forage yield differences were somewhat less.
| Conclusions |
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| NOTES |
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1 Mention of a proprietary product does not constitute a recommendation or warranty of the product by the University of Minnesota and does not imply approval to the exclusion of other suitable products. ![]()
Received for publication January 27, 2000.
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