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a Dep. Zootecnia Ruminantes, Escola Superior de Agricultura "Luiz de Queiroz", Univ. de São Paulo (ESALQ-USP), Caixa Postal 9, Piracicaba, SP 13418-900, Brazil
b Dep. of Agronomy, P.O. Box 110300, Univ. of Florida, Gainesville, FL 32611-0300 USA
c Range Cattle Res. & Educ. Ctr., Univ. of Florida, Ona, FL, 33865 USA
les{at}gnv.ifas.ufl.edu
| ABSTRACT |
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Abbreviations: CP, crude protein GC, grazing cycle IVDOM, in vitro digestible organic matter LAI, leaf area index SH, stubble height
| INTRODUCTION |
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Florakirk (F1 hybrid between `Callie' and `Tifton 44' bermudagrasses) was developed by G.W. Burton at the Coastal Plain Experiment Station in Tifton, GA. It has been evaluated under clipping and grazing in southern Florida as experimental line 35-3 (sometimes called Tifton 35-3 or Callie hybrid 35-3) since 1979 and was released by the Florida Agricultural Experiment Station in 1994 (Mislevy et al., 1995). Another hybrid from the same cross was evaluated as line 35-4 and released as `Tifton 78' because it appeared to have greater rust resistance and lower HCN concentration than 35-3 at the Tifton location (Burton and Monson, 1988).
Tifton 78 is not well adapted to the Florida environment. Adjei et al. (1989) reported poor performance of Tifton 78 and concluded that it was slow to establish and not competitive against weeds during the establishment phase. In the same study, herbage accumulation of Florakirk was superior to Tifton 78, averaging 11.9 Mg ha-1 across grazing frequencies of 2 to 8 wk. Concentration of CP in Florakirk herbage was 152 (2 wk), 100 (4 wk), 91 (6 wk), and 69 g kg-1 (8 wk). Within a grazing frequency, CP was similar to or greater than that of the other warm-season perennial grasses evaluated. In vitro digestible organic matter concentration of Florakirk was similar to that of Tifton 78 at all grazing frequencies, but Florakirk showed the steepest decline of all grasses tested as intervals between grazing events increased from 2 to 8 wk (630 to 440 g kg-1). This was similar to a trend observed by Mislevy et al. (1988).
In several locations in north and north central Florida, Florakirk has been compared under clipping management with commercial bermudagrass cultivars including `Coastal', `Coastcross-1', `Alicia', and Tifton 44 (Mislevy et al., 1995). Productivity and nutritive value responses were equal to or better than those of the other cultivars in most cases. Data are lacking, however, on the responses of Florakirk to grazing management in environments like northern Florida, where frosts and freezes can occur frequently during winter.
Our objective was to evaluate the performance of Florakirk bermudagrass pastures under a range of grazing management treatments in northern Florida. Specific objectives were to measure herbage accumulation and forage nutritive value of Florakirk pastures grazed at different intervals and to different postgraze stubble heights.
| Materials and methods |
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Residue from 1992 growth was burned in March 1993, and in April all pastures were fertilized with 45, 20, and 74 kg ha-1 of N, P, and K, respectively. Rates of P and K used were based on soil test recommendations for improved perennial grass pasture. Pastures received 56 kg N ha-1 as NH4NO3 on 2 June, 21 July, and 1 Sept. 1993. Fifty millimeters of irrigation water was applied on 11 August and 25 mm on 18 August in 1993, because rainfall had been poorly distributed and below average during spring and summer (Table 1). Before grazing started in 1994, pastures were clipped to 10 cm, to remove frosted residue from the previous winter. Fertilization with 45, 20, and 74 kg ha-1 of N, P, and K, respectively, was done on 6 April. Nitrogen was applied at 56 kg ha-1 as NH4NO3 on 11 May, 13 July, and 23 August.
Pastures were sampled to determine herbage mass before and after each grazing, except for GC 7 treatments, which were sampled every 3 wk. Herbage mass was measured using double sampling. At each sampling date, four 0.25-m2 sites per pasture, representing the range of herbage mass, were selected and measured with a disk meter (Santillan et al., 1979). The herbage at the four sites was then clipped at soil level and dried at 60°C in a forced-air dryer to constant weight. The disk meter was also dropped at 30 additional sites within the pasture in a systematic fashion. The same procedure was repeated after grazing. At the end of each grazing season, a calibration equation was developed by regressing herbage mass on disk height using data from all pastures on all dates. The average of the 30 disk heights per pasture was calculated and herbage mass was estimated using the calibration equation. A single equation was used in 1993 and two were used in 1994 (one for GC 7, another for GC 21 and GC 35). Prediction equations had r2 values of 0.745 in 1993, and 0.746 (for GC 7) and 0.591 (for GC 21 and GC 35) in 1994.
Herbage accumulation during a rest period was calculated by subtracting postgraze herbage mass of the previous grazing cycle from pregraze herbage mass of the current cycle. Seasonal herbage accumulation was calculated by adding Cycle 1 pregraze herbage mass and the herbage accumulation values over the remainder of the grazing season for each pasture. For GC 7 pastures, sampling for herbage mass was done every 21 d, although they were grazed weekly to their assigned stubble height. Four caged exclosures in each of these pastures were used to measure herbage accumulation. In these pastures, there was no pregraze herbage mass sampling. After grazing, herbage mass on the pasture as a whole was determined using double sampling, and the herbage inside the four cages (0.25 m2 per cage) was clipped to soil level, dried at 60°C, and weighed. Four new sites for cages were then chosen where stubble height represented that of the pasture. Three weeks later, the same sampling procedure was repeated. Herbage accumulation over the 3-wk period was calculated as the difference between herbage mass inside the cages and herbage mass on the pasture 3 wk earlier.
In order to assess the effects of treatments on forage nutritive value, hand-plucked samples were clipped pregraze to the height of the target stubble height on each sampling date. Within a pasture, 20 samples were cut to make a 200-g fresh weight composite sample. These samples were dried at 60°C in a forced-air dryer to constant weight and ground in a Wiley mill to pass a 1-mm stainless steel screen. Nitrogen concentration was measured using a modification of the aluminum block digestion technique (Gallaher et al., 1975); NH3 in the digestate was determined by semiautomated colorimetry (Hambleton, 1977). Concentration of CP in herbage dry matter was calculated as N x 6.25. In vitro digestible organic matter concentration was determined by the two-stage procedure of Tilley and Terry (1963) modified by Moore and Mott (1974); neutral-detergent fiber determination followed the procedure described by Golding et al. (1985).
To determine the effects of grazing cycle and stubble height across years, data were analyzed using the General Linear Models procedure (PROC GLM) of the Statistical Analysis System software package (SAS Inst., 1989). In this analysis, the grazing treatment combination (GCSH) was the main plot and year was the subplot in a split-plot arrangement. Interactions with year were numerous, so data are reported by year. Within years, data were analyzed using the Response Surface Regression procedure (PROC RSREG) of SAS (SAS Inst., 1989). This procedure tests for fitness of a second-order polynomial regression model of the form
, where y is the response variable, ß0 is the intercept, ß1 is the linear coefficient for GC, ß2 is the linear coefficient for SH, ß3 is the quadratic coefficient for GC, ß4 is the quadratic coefficient for SH, ß5 is the interaction or cross-product coefficient for GC and SH, and
is the experimental error term.
The second-order polynomial model was also tested for lack of fit. Reduced models were fitted with the coefficient estimates that showed a significant effect in the full model (P
0.10), and PROC GLM was used to test for significance (P
0.10) of the coefficient estimates in the reduced models. Terms that were not significant in the full model were included in the reduced model when higher-order terms were significant. For example, when there was a GC x SH interaction, the linear effects of both GC and SH were included in the model, regardless of their level of probability. Likewise, the linear effect of a factor was included when the quadratic effect of that factor was significant. The response surfaces generated are shown as two-dimensional projections on a plane surface (contour plots). When only one factor affected the response, line graphs are used. Coefficient estimates, probability levels for effects of experimental variables and their interactions, and coefficients of determination associated with each fitted model are listed in Table 2
. The P(|t| > t
) =
values are for effects in the reduced model.
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| Results and discussion |
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3 and reduced it to an average of 1.4. In addition, accumulation of dead material occurred below mowing height in weekly-mowed swards. Unlike the responses observed for Coastal by Morgan and Brown (1983a, 1983b), Florakirk herbage accumulation was consistently greater when the grazing cycle was 7 d than when it was 21 d for a given level of stubble height. For frequently and closely grazed swards, it is likely that the Florakirk canopy had a higher tiller density (not measured) and thus was more efficient in intercepting incoming radiation than when grazing cycle was intermediate (e.g., 21 d). Stimulation of tillering by increased grazing intensity has been documented by several authors (e.g., Langer, 1963; Jewiss, 1972; Kays and Harper, 1974). Matthew et al. (1995) and Sackville Hamilton et al. (1995) have described this response as the "-3/2 self-thinning rule." According to those authors, in response to increasing defoliation intensity, reduced tiller weight is compensated by increased tiller population density. Binnie and Chestnutt (1991) found evidence of an increase in tiller number when they reduced the regrowth interval of perennial ryegrass (Lolium perenne L. cv. Talbot) from 4 to 3 wk. In the mob-grazing trial reported here, pastures grazed every 7 d to 8 cm appeared on visual examination to have greater ground coverage, with plants growing more prostrate, almost turf-like, and probably with more tillers per unit area. In this situation, the continuous emergence of new tillers may have contributed substantially to interception of a high proportion of light by young, photosynthetically active tissue and thus increased C assimilation via photosynthesis. Hodgson (1990) explained that the decline in the rate of net C assimilation with defoliation is not as great on continuously stocked or very frequently cut swards as it is on less frequently swards cut or grazed swards. He attributes this response to high tiller populations and large numbers of young leaves which, because they have developed in bright light, have a high photosynthetic potential.
As the grazing cycle increased from 7 to 21 d in the current study, there was probably less stimulus to tillering. Carbon uptake per unit land area may therefore have been reduced, resulting in lower herbage accumulation when the grazing cycle was 21 d. Increased herbage accumulation when the grazing cycle was increased to 35 d is probably related to optimization of LAI. In other words, tiller density was probably lower than in GC 7 treatments, but the longer regrowth interval afforded the plants time to increase LAI and grow for an extended period at optimal LAI. The proportion of leaf tissue removed by grazing may have been greatest for GC 21 treatments, because stimulation of basal tillering and the development of leaf area close to soil surface was not as great as in GC 7 plots and, at the same time, there was not as much time for leaf development as in GC 35 treatments.
In addition to the physiological mechanisms already described, the cage technique used to quantify herbage accumulation in this study may have contributed in part to higher herbage accumulation for GC 7. On these pastures, herbage accumulation was measured in caged areas and the cages were rotated every 21 d. This length of time was necessary to avoid clipping a large proportion of the pasture area over the course of the season, something that would have occurred with weekly sampling. Inflated herbage accumulation within cages was reported by Cowlishaw (1951) and Heady (1957), and explained on the basis of a modified environment within the cage, including increased humidity, decreased wind velocity, and lower transpiration losses. In addition, plants inside cages probably benefited from greater leaf area than those outside during the 3-wk exclusion period (Heady, 1957) and there was no damage by animal treading or fouling within the caged exclosure (Cowlishaw, 1951).
The increase in herbage accumulation associated with increasing stubble height has been addressed by Parsons (1988). He explained that, in swards maintained at high LAI (i.e., taller swards), high rates of respiration result from the greater gross photosynthesis and the greater weight of live tissue to be maintained. Although the absolute amount of dry matter lost in respiration is greater in swards of high rather than low LAI, in both cases the proportion of gross photosynthesis consumed by this route is the same (about 45%) (Parsons et al., 1983). This means that, in effect, shaded leaves respire less than illuminated leaves and, as a result, the gross rate of shoot production is greater in a sward maintained at a high LAI than in a sward maintained at a low LAI (Parsons, 1988).
Herbage Nutritive Value of Hand-Plucked Samples
Range in herbage CP and IVDOM was relatively small, but both were affected by grazing treatment. Crude protein concentration was not affected by stubble height in either year (P > 0.20 in 1993; P > 0.57 in 1994), but there were linear and quadratic effects of grazing cycle in both years (Fig. 2)
. From short (7 d) to intermediate (21 d) levels of grazing cycle, there was a slight increase in CP. This was followed by a decline in CP concentration reaching a minimum at GC 35. Even so, the range in herbage CP concentration was less than 18 g kg-1 in both years (96 to 113 g kg-1 in 1993 and 124 to 133 g kg-1 in 1994). Figure 2 shows that the maximum CP concentration is predicted to occur when the grazing cycle is less than 21 d, regardless of the height of the stubble left after grazing.
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affecting IVDOM concentration (Fig. 3)
. There was little effect on IVDOM from varying stubble height at GC 7. Increasing stubble height at GC 35, however, caused a decline in IVDOM concentration from a maximum of 650 g kg-1 in the GC 35SH 8 treatment, to a minimum of 598 g kg-1 in the GC 35SH 24 treatment. A different response was observed in 1994, when stubble height did not (P > 0.62) affect IVDOM concentration. Similar to CP concentration, there were both linear and quadratic effects of grazing cycle on IVDOM. Intermediate levels of grazing cycle tended to maximize IVDOM, with declines at both ends of the grazing cycle scale. Whereas IVDOM concentration peaked at 592 g kg-1 for GC 21, it was 565 and 569 g kg-1 for GC 7 and GC 35, respectively. Concentration of neutral-detergent fiber in the herbage did not respond to grazing treatments in 1993
or
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In 1993, there was a GC x SH interaction effect on IVDOM. When the grazing cycle was long, taller stubble heights were detrimental to digestibility, perhaps because a greater proportion of the regrowth was made up of stem, which reduces digestibility. Reduced digestibility of Coastal bermudagrass stem was attributed by Akin et al. (1990) to increased presence of lignified ring tissues when plants are 6 wk old. This effect of stubble height was not observed in the short-GC treatments. This is consistent with the notion that young regrowth present at short grazing cycles, regardless of stubble height, consists mainly of high nutritive value leaf and young stem tissue.
| Conclusion |
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| NOTES |
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Received for publication September 11, 1998.
| REFERENCES |
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