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Irrigation to Maximize Vaccine Antigen Production in Genetically Modified Tobacco

^a Univ. of Missouri-Delta Research Center, P.O. Box 160, Portageville, MO 63873
^b USDA Cropping Systems and Water Quality Research Unit, Portageville, MO 63873
^c Chlorogen, Inc., 893 North Warson Road, St. Louis, MO 63141

View larger version (53K): [in this window] [in a new window]   Fig. 1. Diagram of the arrangement of each plot for the irrigation experiment with soil in a glasshouse at Portageville, MO. Rainbird (Azusa, CA) XS-090 irrigation sprinklers were placed in opposite ends of each plot and adjusted to distribute water evenly without over-spraying plots. A narrow trench/dike was constructed around the border to contain run-off water and discourage root encroachment from neighboring plots. Watermark (Irrometer, Inc., Riverside, CA) soil moisture sensors were buried 10 cm deep in the center of each plot.

View larger version (13K): [in this window] [in a new window]   Fig. 2. Soil water weight loss from evapotranspiration during 5 d without watering from pots growing protective antigen tobacco and Petite Havana (nontransgenic parent) plants. Bars indicate standard error mean.

View larger version (12K): [in this window] [in a new window]   Fig. 3. Transpiration rate per leaf area during 5 d without watering from pots growing protective antigen tobacco and Petite Havana (nontransgenic parent) plants. Measurements were made with a portable photosynthesis system. Bars indicate standard error mean.

View larger version (21K): [in this window] [in a new window]   Fig. 4. SPAD chlorophyll meter readings from youngest developed leaf (fourth from apex) of protective antigen tobacco as affected by irrigation threshold based on soil water potential. Leaf measurements were made before both harvests. Points are means from four replicated plots with tobacco grown on Tiptonville sandy loam soil. Bars indicate standard error mean.