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Proteolysis and Characterization of Peptidases in Forage Plants

Facultad de Agronomía e Ingeniería Forestal, Pontificia Universidad Católica de Chile, Vicuña Mackenna 4860, Macul, Santiago, Chile

View larger version (10K): [in a new window]   Fig. 1. Schematic representation of tools used for mechanical damage of the leaf discs: (a) glass plate; (b) channel, 0.5-mm width; (c) leaf disc, 3-mm diam.; (d) sintered glass rod, 5-mm diam.; and (e) weight, 400 g.

View larger version (76K): [in a new window]   Fig. 2. Petri dishes with agar-gelatin films: (a) Petri dish with samples and papain standards before incubation and (b) Petri dish after peptidase action showing spots with variable activities over a dark background. () Papain standards.

View larger version (52K): [in a new window]   Fig. 3. Protein degradation of forage genera in agar-gelatin films, expressed as proteolytic activity index (PAI). Lines over the bars represent standard error of the estimated mean.