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Differential Nitrogen-15 Labeling of Dairy Manure Components for Nitrogen Cycling Studies

J. Mark Powell*,a, Zhiguo Wub, Keith Kellingc, Paul Cusickc and Gabriela Muñozc

a USDA-ARS, Dairy Forage Res. Cent., 1925 Linden Drive West, Madison, WI 53706
b Dep. of Dairy and Anim. Sci., Pennsylvania State Univ., 324 William L. Henning Bldg., University Park, PA 16802
c Dep. of Soil Sci., Univ. of Wisconsin–Madison, 1525 Observatory Drive, Madison, WI 53706



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Fig. 1. Differential 15N labeling of urine N and fecal N components by feeding 15N-enriched forage or 15N-enriched urea to dairy cows.

 


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Fig. 2. Nitrogen-15 concentration in urine of two dry dairy cows after feeding 15N-enriched forage. (Base of arrows marks the time when last offer of 15N-enriched forage was made.)

 


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Fig. 3. Nitrogen-15 concentration in feces of two dry dairy cows after feeding 15N-enriched forage. (Base of arrows marks the time when last offer of 15N-enriched forage was made.)

 


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Fig. 4. Nitrogen-15 concentration in urine and feces of two dry dairy cows after feeding 15N-enriched urea. (A single dose of 15N-enriched urea was fed in 1999, and eight doses were fed in 2000.)

 


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Fig. 5. Atom% 15N in total fecal N and fecal undigested feed N of two dry dairy cows fed 15N-enriched forage. (Base of arrows marks the time when last offer of 15N-enriched forage was made.)

 





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