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A Sampling Method for Measurement of Large Root Systems with Scanner-Based Image Analysis

Carlos Costaa, Lianne M. Dwyerb, Robert I. Hamiltonb, Chantal Hamelc, Line Nantaisc and Donald L. Smithc

a Univ. of Passo Fundo, Passo Fundo, RS, 99001-970, Brazil
b Agriculture and Agri-Food Canada, Ottawa, Eastern Cereal and Oilseed Research Centre, Ottawa, ON, Canada
c Dep. of Plant Science, McGill Univ., Macdonald Campus, 21,111 Lakeshore, Ste-Anne-de-Bellevue, Quebec, Canada H9X 3V9



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Fig. 1 Side and top views of the air-stirring–based device for randomly mixing roots

 


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Fig. 2 Effectiveness of lettuce spinner in removing excess water from root subsamples as a function of spinning time (R2 = 0.99, P < 0.05)

 


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Fig. 3 Relationship between spun fresh weight and root length of maize genotypes

 


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Fig. 4 Effect of measured root subsample size on the number of root crossovers (using 200 by 300 mm tray size). Bars extending above and below each point indicate ±1 SE

 


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Fig. 5 Mean root lengths of the randomly collected subsamples (0.5 g fresh wt.) from the mixing device. Bars extending above and below each point indicate ±1 SE

 


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Fig. 6 Sample size as determined by standard parametric and nonparametric (bootstrap) procedures

 


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Fig. 7 Relationship between sample size and half-length 95% confidence intervals for root length of three genotypes and for the mean of all genotypes

 


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Fig. 8 Relationship between total dry masses and total root length of three maize genotypes. Each point represents the overall mean for a given genotype

 





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