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Methods for Creating Stomatal Impressions Directly onto Archivable Slides

^a USDA-ARS, 3810 4th Street, Lubbock, TX 79415-3397
^b USDA-ARS, Cropping Systems Research Laboratory, Big Spring, TX 79720-0013

View larger version (180K): [in this window] [in a new window]   Fig. 1. Brightfield micrographs of epidermal impressions made with various polymer and solvent systems. (A) Cellulose acetate (CA)/acetone impression of adaxial (upper) tobacco leaf epidermis, (B) Cellulose acetate butyrate (CAB)/acetone impression of abaxial (lower) tobacco leaf epidermis, (C) Polymethyl methacrylate (PMMA)/methylene chloride (MeCl2) impression of adaxial sorghum epidermis, (D) CAB/acetone impression of adaxial sorghum epidermis, (E) PMMA/MeCl2 impression of eastern gamagrass epidermis, (F) CAB/acetone impression region around vein of abaxial tomato leaf.