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Agronomy Journal 93:590-597 (2001)
© 2001 American Society of Agronomy

CORN

Comparison of Late-Season Diagnostic Tests for Predicting Nitrogen Status of Corn

Richard H. Fox, William P. Piekielek and Kirsten E. Macneal

Dep. of Agron., 116 ASI Building, Penn State Univ., University Park, PA 16802

Corresponding author (rhf{at}psu.edu)

Received for publication July 14, 2000. We compared six late-season diagnostic tests for determining N adequacy in corn (Zea mays L.) in a 3-yr study in Pennsylvania. The six tests were: (i) the NO-3–N concentration of stalk sections at black layer; (ii) the NO-3–N concentration of stalk sections at the one-fourth milk line growth stage (MLGS), which allows corn grown for silage to be tested; (iii) the chlorophyll meter (CM) test at the one-fourth MLGS; (iv) the relative CM test (normalized values) at the one-fourth MLGS; (v) a visual test based on the number of green leaves below and including the ear leaf at the one-fourth MLGS; and (vi) a relative visual test (normalized values at the one-fourth MLGS). We found that with a critical level of 250 mg kg-1 NO-3–N, the stalk NO-3 test separated N-sufficient from N-deficient sites with approximately 93% accuracy when sampling was done at either the one-fourth MLGS or within several weeks after black-layer formation. It appears that the 250 mg kg-1 NO-3–N critical level can be used to accurately predict N adequacy for any sampling time between the one-fourth MLGS and a few weeks after black-layer formation. When drought-stressed fields were excluded or the CM readings normalized with a high-N reference plot in the field, the accuracy of the CM test at the one-fourth MLGS was approximately 92%. The visual test at the one-fourth MLGS was an accurate predictor of corn N status only when visual readings were normalized with a high-N reference plot. These results demonstrate that there are several late-season N tests that are suitable for making relatively accurate assessments of N sufficiency for corn silage and grain yields.

Abbreviations: CM, chlorophyll meter • gl/p, green leaves per plant • MLGS, milk line growth stage




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