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NOTES & UNIQUE PHENOMENA

Digital Acquisition and Measurement of Peanut Root Minirhizotron Images

Dep. of Crop and Soil Sciences, The University of Georgia, 1109 Experiment St., Griffin, GA 30223-1797 USA

gpatena{at}arches.uga.edu

Video, photo, or in situ image capture and analysis from minirhizotrons is time-consuming. Computerized root image analysis is expensive and subject to large background error. This paper describes a fast, simple, and accurate method for (i) capturing minirhizotron images through low-cost hardware upgrade of existing minirhizotron equipment, and (ii) processing digital images using low-cost software. This system was developed to monitor root distribution in peanut (Arachis hypogaea L.). Digital images were recorded from a minirhizotron camera directly to a laptop computer hard drive instead of using videotape. This reduced image acquisition time by 36% and eliminated problems associated with manipulating video players. The image analysis system uses a mouse pointer to trace root length and diameter on a computer screen and calculates the distance traversed by the mouse pointer. A paired t-test on accuracy using wires of known length showed no significant difference (P = 0.10) between actual and measured lengths.

Abbreviations: BMP, bitmap graphics file • GIF, graphics interchange format • JPEG, joint photographic expert group • NTSC, National Television Standards Committee • PAL, phase alternating line • PCI, peripheral component interconnect • PP-VCD, parallel port video capture device • SECAM, sequential color and memory • TIFF, tagged image file format • VHS, video home system

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