Agronomy Journal Journal of Natural Resources and Life Sciences Education
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Published online 1 September 1993
Published in Agron J 85:987-990 (1993)
© 1993 American Society of Agronomy
677 S. Segoe Rd., Madison, WI 53711 USA
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Adjustment for Specific Leaf Weight Improves Chlorophyll Meter's Estimate of Rice Leaf Nitrogen Concentration

Shaobing Peng*, Felipe V. García, Rebecca C. Laza and Kenneth G. Cassman

Agronomy Plant Physiology, and Agroecology Div., IRRI( Int. Rice Res. Inst.), P.O. Box 933, 1099 Manila, Philippines

* Corresponding author

The chlrophyll meter provides a simple, quick, and nondestructive method to estimate leaf N status of rice (Oryza sativa L.), but the linear relationship between leaf N concentration of a dry-weight basis (Ndw) and the meter reading differs depending on a developmental stage and genotype. The objective was to determine whether prediction of (Ndw) with the chlorophyll meter can be improved by a simple correction for specific leaf weight (SLW). Leaf N status was estimated by a chlorophyll meter (SPAD-502) and measured directly by micro-Kjel-dahl procedure. specific leaf weight was calculated as the ratio of dry weight to leaf area. In one field study ‘IR72’, measurements were taken at midtillering, panicle initiation, and flowering stages on the uppermost fully expanded leaves of both N-deficient and N-sufficient plants. There was a liner relationship between Ndw> and spad values at each stage, but regression lines differed significantly between growth stages. Based on pooled data from all stages, the degree of linear fit was poor (r2 = 0.49). Adjusting SPAD values for SLW (SPAD/SLW) improved the prediction of Ndw (r2 = 0.93). For another setof measurement made on the flag leaves of five genotypes grown in the field and greenhouse, prediction of Ndw was also improved, from r2 = 0.51 based on SPAD values alone to r2 = 0.87 based on the SPAD/SLW ratio. These results demonstrate that SLW influences the prediction of Ndw by the chlorophyll meter, and that the adjustment of SPAD valuers for SLW greatly increases the accuracy of the prediction. However, when SPAD values are adjusted for SLW, the chlorophyll meter's estimate of N dw is no longer as quick, simple, or nondestructive as the nonadjusted SPAD values.

Contribution from IRRI.

Received for publication October 28, 1992.


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