HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ashworth, E. N. Articles by Oberdorf, R. L. Search for Related Content PubMed Articles by Ashworth, E. N. Articles by Oberdorf, R. L. Agricola Articles by Ashworth, E. N. Articles by Oberdorf, R. L.

Imbibitional Chilling Injury in Soybean Axes: Relationship to Stelar Lesions and Seasonal Environments¹

The increasing use of isolated embryonic axes as experimental issues for studies on early germination events warrants an evaluation of stress effects during hydration. The objectives were to evaluate embryonic axis growth and stress crack formation in soybean (Glycine max (L.) Merr.) seeds and isolated axes resulting from hydrational stresses of cold temperatures and low tissue moisture. Sensitivity to imbibitional chilling of seeds and isolated axes was evaluated in several cultivars produced in different years at different locations. Germination, seedling growth, and formation of stelar lesions were measured. Isolated axes with an initial moisture content of 6% were injured by imbibition at 5 C as evidenced subsequently by reduced growth at 25 C. Adjustment of axis moisture to 17% before hydration protected against imbibitional chilling injury. Sensitivity to imbibitional chilling was influenced by cultivar and year or location of production. Lesions formed across the stele of hypocotyls during rapid hydration of 6% initial moisture isolated axes at 5 or 25 C but were not observed after hydration of 17% moisture axes or after hydration of intact seeds. The presence of lesions suggests caution when using isolated embryonic axes as experimental tissues.

Key Words: Glycine max (L.) Merr. • Seedling vigor • Maternal environment

² Graduate research assistant (now plant physiologist, USDA, SEA-AR, Appalachian Fruit Research Station, Route 2 Box 45, Kearneysville, WV 25430) and professor of crop science, respectively.

Received for publication August 25, 1978.

This article has been cited by other articles:

				M. Koizumi, K. Kikuchi, S. Isobe, N. Ishida, S. Naito, and H. Kano Role of Seed Coat in Imbibing Soybean Seeds Observed by Micro-magnetic Resonance Imaging Ann. Bot., September 1, 2008; 102(3): 343 - 352. [Abstract] [Full Text] [PDF]