HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Graham, P. H. Articles by Duque, A. Search for Related Content PubMed Articles by Graham, P. H. Articles by Duque, A. Agricola Articles by Graham, P. H. Articles by Duque, A.

Survival of Rhizobium phaseoli in Contact with Chemical Seed Protectants¹

In the multiple cropping systems used for bean (Phmeolus vulgaris L.) production in Latin America, seed treatment Is often needed for control of soil pathogens and pests, but can be incompatible with Rhizobium inoculation. This study looks at the interaction between Rhizobium and seed protectant. One laboratory and three field studies are reported. The survival of R. phaseoli on bean seeds treated with PCNB, thiram, or captan was measured under laboratory conditions using standard serial dilution and plate count techniques. At Popayan, Colombia (typic dristandept, pH 4.8) the nodulation and nodule development of bean seeds treated with PCNB, thiram, captan, and furadan was evaluated, and different inoimlation methods, including a granular soil applied inoculant, compared. Nodulation of beans protected with PCNB or thiram, and inoculated with seed or soil-applied rhizobia was also studied at La Selva, Colombia (typic dristandept, pH 5.1).

While contact with PCNB, thiram or captan on seeds reduced Rhizobium survival, with captan most toxic, PCNB-treated seeds maintained counts of l0³ rhizobialg seed until almost 48 hours after inoculation. Field results demonstrated the delicate balance between inoculant and seed protectant. At Popayan, nodule number/plant was not affected when thiram, furadan, or PCNB-treated seeds were inoculated and promptly planted, but delay between inoculation and planting did reduce the nodulation of seeds protected with PCNB or thiram. At La Selva, all fungcide treatments lowered nodule number/plant. Seed protection with PCNB enhanced nodule development at Popayan and reduced root rot incidence at La Selva. Despite the somewhat acid soil conditions the best nodulation at both Popayan and La Selva was attained with granular soil-applied inoculants. Further studies on this method of inoculation for beans are warranted.

Key Words: Inoculation • Granular inoculum • Fungicides • Nodulation • Pelleting

² Microbiologist, research technician, research assistant, and research assistant, respectively.

Received for publication September 5, 1979.

This article has been cited by other articles:

				H. H. Zahran Rhizobium-Legume Symbiosis and Nitrogen Fixation under Severe Conditions and in an Arid Climate Microbiol. Mol. Biol. Rev., December 1, 1999; 63(4): 968 - 989. [Abstract] [Full Text] [PDF]