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Comparison of Cottonseed Delinting Methods in Evaluating Seed-treatment Fungicides¹

Evaluating fungicides used to treat planting seed of cotton (Gossypium hirsutum L.) had traditionally been made with acid-delinted seed and repeated on machinedelinted seed under the assumption that the treatment response may depend upon the delinting method used. Machine and acid-delinted cottonseed from the same seed lot were treated with different fungicides. The seed were planted in replicated field tests at eight locations for 3 years. Stand counts were recorded about 30 days after planting. Our primary concern was to determine the effects of methods of delinting cottonseed so seedling survival, but the effects of seed treatments, locations, and years and their interactions were determined. There were no differences in mean percentages of survived seedlings between acid and machine-delinted cottonseed and there were no significant interactions of deliiting method and any of the other variables except those noted below. Differences in seedling survival among years and locations were not significant, but the first-order interaction between these components was significant, indicating a need to test fungicides each year at as many locations as is feasible. The results should be the same for acid-delinted as for machine-delinted cottonseed. There were stand differences among the seed treatments, but most of these differences were between fungicide-treated seed and the untreated seed. Small differences occurred in percentage of seedling survival among the fungicide seed treatments. Either acid or machine-delinted cottonseed can be usd to evaluate fungicide seed treatments for their effect on plant stands. Acid-delinted seed should be used in these tests since most of the planting seed are acid-delinted.

Key Words: Fungicide • Gossypium hirsutum L. • Statistic • Stands • Seedling disease

² Research plant pathologist and research geneticist, USDA, SEA, AR, Cotton Research Laboratory, Route 3, Lubbock, TX 79401. Present address of the first author is Cotton Physiology and Genetics Laboratory, P. 0. Box 225, Stoneville, MS 38776.

Received for publication July 23, 1979.