Agronomy Journal Journal of Natural Resources and Life Sciences Education
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Published online 1 July 1976
Published in Agron J 68:686-690 (1976)
© 1976 American Society of Agronomy
677 S. Segoe Rd., Madison, WI 53711 USA
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vogel, K. P.
Right arrow Articles by Lenser, G. W.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Vogel, K. P.
Right arrow Articles by Lenser, G. W.
Agricola
Right arrow Articles by Vogel, K. P.
Right arrow Articles by Lenser, G. W.

Modified Milling Procedure for Separating Endosperm and Nonendosperm Portions of the Wheat Kernel for Protein and Lysine Analysis1

K. P. Vogel, P. J. Mattern and G. W. Lenser2

Conventional milling does not uniformly separate endosperm and nonendosperm components of wheats (Triticum aestivum L.). Uniform separation of bran and endosperm is required for protein and lysine analyses because of a protein gradient in wheat endosperm. A modified milling procedure for separating endosperm and nonendosperm components of the wheat kernel was evaluated for its utility in processing small breeder lots of seed for protein and lysine analyses. After conventional milling and sifting, the endosperm adhering to the bran was removed by washing with a 80:20 (vol/vol) ethanol:acetone solution, filtered from the washing solution, dried, and added to the mill flour to reconstitute the endosperm. Reconstituted endosperm weight was used to calculate percent endosperm. Reasonably complete and uniform separations of the endosperm and nonendosperm components were obtained without any appreciable loss of components. Endosperm percentages of the wheats tested ranged from 72.8 to 85.5%. Endosperm protein percentages ranged from 10.8 to 21.0%. Bran protein percentages ranged from 13.1 to 25.1%. Endosperm lysine (percent of protein) percentages ranged from 2.1 to 3.1%. Bran lysine (percent protein) percentages ranged from 3.2 5.0%.

Key Words: Triticum aestivum L. • Wheat quality • Wheat bran

1 Contribution of the Dep. of Agronomy, Univ. of Nebraska- Lincoln, and the ARS, USDA, Lincoln, Neb. Part of a dissertation submitted by the senior author to the Graduate College of the Univ. of Nebraska-Lincoln in partial fulfillment of the Ph.D. degree. The research was supported in part by funds from the Agency for International Development, U. S. Dep. of State, Washington, DG Contract Nos. AID/csd-1208 and AID/ ta-c 1903. Published as Paper No. 4040, Journal Series, Nebraska Agric. Exp. Stn.

2 Research agronomist, ARS, USDA, formerly NDEA Title IV fellow, Univ. of Nebraska-Lincoln; professor (cereal quality), Univ. of Nebraska-Lincoln; and manager, Quality Assurance Feed Division, ConAgra, Omaha, Neb.

Received for publication September 27, 1975.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
The SCI Journals Crop Science Vadose Zone Journal
Journal of Natural Resources
and Life Sciences Education		 Soil Science Society of America Journal
Journal of Plant Registrations Journal of
Environmental Quality		 The Plant Genome
Copyright © 1976 by the American Society of Agronomy.