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Detection of Iron Ethylenediamine Di(O-Hydroxyphenylacetate) in Plant Tissue¹

A method was developed for the extraction of iron ethylenediamine di(o-hydroxyphenylacetate) (FeEDDHA) from plant tissue and the technique was used to study the absorption of FeEDDHA by plants. A published method for the extraction of FeEDDHA was modified in an attempt to eliminate plant components which interfered with the spectrophotometric determination of the iron chelate. The method which was developed involves the use of lead acetate and ammoniums ulfate to precipitate interfering substances. The FeEDDHA is then extracted into -amyl alcohol.

Tobacco plants were exposed to FeEDDHA at various concentrations and for various periods of time. The leaves of the plants were subsequently analyzed to determine whether they contained detectable amounts of the intact iron chelate. The results suggested that FeEDDHA accumulated in the leaves of plants which had been grown on the high levels of the iron chelate or which had been grown on FeEDDHA for long periods of time.

Key Words: chelates • FeEDDHA • iron • chelate absorption

² Department of Agricultural Sciences, University of California, Los Angeles 90024.

Received for publication March 4, 1968.

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