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Published in Agron J 59:473-474 (1967)
© 1967 American Society of Agronomy
677 S. Segoe Rd., Madison, WI 53711 USA
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Selection of Index Leaf for Studying the Critical Concentration of Nitrogen in Rice Plants1

E. F. Wallihan and J. C. Moomaw2

Changes in nitrogen (N) concentration in the last four leaves formed on rice (Oryza sativa L.) culms of three varieties growing in paddy in the Philippines during the rainy season were studied during the period from formation of flower primordia until after flower emergence. On the basis of stability of N concentration and sensitivity to N supply, the blade of the next-to-last leaf (second leaf below the panicle), excised at the time of flower emergence, was chosen as the index leaf.

Key Words: rice • index leaf • critical concentration • nitrogen in rice plants


1 Contribution from the International Rice Research Institute, Los Banos, Laguna, The Philippines. The authors are indebted to Dr. Akira Tanaka for providing leaf samples from his field plots, to J. Lapis and L. Lucas for able assistance in collecting and preparing the samples, and to A. P. Tianco for the chemical analyses.

2 Visiting Plant Physiologist (present address: Department of Soils and Plant Nutrition, University of California, Riverside 92502) and Agronomist.

Received for publication April 24, 1967.





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The Plant Genome
Copyright © 1967 by the American Society of Agronomy.