Agronomy Journal Journal of Natural Resources and Life Sciences Education
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Published online 1 January 1966
Published in Agron J 58:13-16 (1966)
© 1966 American Society of Agronomy
677 S. Segoe Rd., Madison, WI 53711 USA
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Influence of Drying Method and Fertility Treatments on the Total and Water Soluble Nitrogen Contents of Alfalfa1

J. C. Burns, C. H. Noller and C. L. Rhykerd2

An established stand of Ranger alfalfa was fertilized with various combinations of nitrogen, phosphorus, and potassium. One sample of freshly harvested material was immediately immersed in liquid nitrogen, freeze-dried, and stored in a refrigerator. A second sample was weighed" and dried to a constant weight. Forage was analyzed for total nitrogen and water soluble nitrogen (WSN).

Freeze-dried forage was found to be significantly higher (P<.01) in WSN but significantly lower (P<.01) total nitrogen than the oven-dried material. Total nitrogen was not significantly affected by fertilizer treatments in the freeze-dried forage. However, harvest date had a significant effect. Oven-dried forage showed both a significant fertilizer and harvest effect.

The WSN content of forage subjected to freeze-drying was not altered significantly by either fertilizer treatments or harvest date. Identical samples of oven-dried material showed contrary results with significant effects for both fertilizer treatments and harvest date. The variance estimates showed chemical analyses from the freeze-dried forage to be more variable than similar oven-dried material.

Since the method of drying had a definite effect on the total and water soluble nitrogen constituents of forage plants, analyses on oven-dried samples may not be representative of green plant material.

1 Contribution from Indiana Agricultural Experiment Station, Departments of Agronomy and Animal Sciences, Lafayette, Indiana. Journal Paper No. 2545. Acknowledgment is made to International Minerals and Chemical Corporation and the American Potash Institute, Inc. for support of this project.

2 Graduate Research Instructor of Agronomy, Professor of Animal Sciences, and Associate Professor of Agronomy, Purdue University. Lafayette, Indiana.

Received for publication May 17, 1965.




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Journal of Natural Resources
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Environmental Quality		 The Plant Genome
Copyright © 1966 by the American Society of Agronomy.