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Crossing of Smooth Bromegrass, Bromus inermis Leyss, in the Greenhouse¹

Sypnosis: Single-crosses in smooth bromegrass can be obtained in the greenhouse by combining hot-water emasculation with open pollination. All clones in a group are emasculated except the pollen parent. Groups can be isolated by placing each on long-days at intervals of about 2 weeks. Immersing slightly immature panicles in water at 46° C. for 5 minutes provided effective emasculation. Maximum panicle production was obtained after 3 months of short-days in a cool greenhouse. Cross-fertility in the greenhouse was positively correlated with selffertility in the field. Yields of single-cross seed were increased by spraying the seed-parents with a fine mist at flowering.

² Formerly graduate assistant, Department of Agronomy, University of New Hampshire, now graduate assistant, Department of Agronomy, University of Wisconsin; and associate professor, Department of Agronomy, University of New Hampshire.

Received for publication January 18, 1957.