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NOTES & UNIQUE PHENOMENA

Methods for Creating Stomatal Impressions Directly onto Archivable Slides

^a USDA-ARS, 3810 4th Street, Lubbock, TX 79415-3397
^b USDA-ARS, Cropping Systems Research Laboratory, Big Spring, TX 79720-0013

^* Corresponding author (dennis.gitz{at}ars.usda.gov).

Stomatal density has been shown to be a primary determinant of crop yield, water use efficiency, and limitation to CO₂ assimilation rate. Widely used methods of assessing stomatal density sample relatively small regions of the leaf, are labor intensive, or do not yield stable archivable samples for revisiting samples. We describe several methods of producing such epidermal impressions that yield samples large enough to generate stomatal density maps across entire leaf surfaces.

Abbreviations: CA, cellulose acetate • CAB, cellulose acetate butyrate • MeCl₂, methylene chloride • MEK, methylethyl ketone • PMMA, polymethyl methacrylate • PVC, polyvinyl chloride

¹ Mention of this or other proprietary products is for the convenience of the readers only, and does not constitute endorsement or preferential treatment of these products.

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Received for publication May 2, 2008.